MEDIATORLESS HYDROGEN-PEROXIDE ELECTRODE BASED ON HORSERADISH-PEROXIDASE ENTRAPPED IN POLY(O-PHENYLENEDIAMINE)
MEDIATORLESS HYDROGEN-PEROXIDE ELECTRODE BASED ON HORSERADISH-PEROXIDASE ENTRAPPED IN POLY(O-PHENYLENEDIAMINE)
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DOI：
10.1016/0022-0728(94)03465-6
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发表时间：
1994-10-31
影响因子：
4.5
通讯作者：
DONG, SJ
中科院分区：
文献类型：
--
作者：
DENG, Q;DONG, SJ
描述了一种基于辣根过氧化物酶 (HRP) 和邻苯二胺 (o-PD) 共电聚合的无介质 H2O2 传感器。该电极在几秒钟内对 H2O2 做出响应，并在 -100 mV 下给出 73.3 nA 1 mu mol(-1) cm(-2) 的电流密度（相对于 Ag\AgCl）。在 1-130 μmol l(-1) 范围内获得线性校准曲线。从动力学分析来看，生物传感器的响应速率受到酶动力学的限制，表观米氏常数K'(m)为220 μ mol l(-1)。由聚邻苯二胺薄膜中包埋的 HRP 组成的电极表现出良好的稳定性，因为 HRP 和邻苯二胺之间在电聚合过程中存在一定的相互作用。
A mediatorless H2O2 sensor based on coelectropolymerization of horse radish peroxidase (HRP) and o-phenylenediamine (o-PD) is described. The electrode responds to H2O2 in a few seconds and gives a current density of 73.3 nA 1 mu mol(-1) cm(-2) at -100 mV (versus Ag\AgCl). A linear calibration curve is obtained over the range 1-130 mu mol l(-1). From kinetic analyses, the response of the biosensor is rate limited by the enzyme kinetics, and the apparent Michaelis-Menten constant K'(m) is 220 mu mol l(-1). The electrode consisting of HRP entrapped in poly(o-phenylenediamine) film shows good stability because there is some interaction between HRP and o-PD in the electropolymerization.