Alkali-soluble polysaccharides of Rhizoclonium riparium alga induce IL-1 gene expression via protein kinase signaling pathways
Alkali-soluble polysaccharides of Rhizoclonium riparium alga induce IL-1 gene expression via protein kinase signaling pathways
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DOI：
10.1021/jf060442f
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发表时间：
2006-05-17
影响因子：
6.1
通讯作者：
Chao, Louis Kuoping
中科院分区：
文献类型：
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作者：
Hsu, Hsien-Yeh;Hua, Kuo-Feng;Chao, Louis Kuoping
用各种藻类强化水产养殖食品可以提高某些鱼或虾对疾病的抵抗力，并且作为常规程序，已经变得越来越受欢迎，并且似乎很重要。本文中，我们从Rhizoclonium riparium Riparium（RASP）中分离出某些碱溶性多糖，这些多糖可以根据多糖的分子量分成两个不同的组。通过气相色谱-质谱联用分析，我们发现组成RASP高分子量组的主要单糖是半乳糖（41.99%）、葡萄糖（34.53%）、木糖（20.24%）和甘露糖（3.24%）。使用小鼠源性巨噬细胞系J774A.1，我们发现，多糖成分的高分子量组的RASP能够诱导白细胞介素-1 β（IL-1）基因表达通过蛋白激酶介导的信号转导途径。实质上，我们发现c-Jun N-末端激酶（JNK）和p38丝裂原活化蛋白激酶（p38），而不是细胞外信号调节激酶（ERK），在RASP刺激的J774A.1细胞中IL-1基因表达的调节中起重要作用。据我们所知，这是第一次从R.已证明利帕铵通过诱导巨噬细胞内的IL-1发挥免疫调节特性。我们目前的结果为R的可能用途提供了支持。Riparium作为各种食物/食品的添加剂，以调节人或某些动物的免疫反应。
Fortification of aquaculture foodstuff with various algae may improve the resistance of certain fish or shrimp to diseases and, as a routine procedure, has become ever more popular and, seemingly, important. Herein, we isolated certain alkali-soluble polysaccharides from a Rhizoclonium riparium alga (RASP), polysaccharides that can be separated into two different groups on the basis of the polysaccharide's molecular weight. Using gas chromatography-mass spectometry analysis, we found that the major monosaccharides constituting the higher molecular-weight group of RASP were galactose (41.99%), glucose (34.53%), xylose (20.24%), and mannose (3.24%). Using a murine-derived macrophage cell line J774A.1, we found that polysaccharide constituents of the higher molecular-weight group of RASP were able to induce interleukin-1beta (IL-1) gene expression via protein kinase-mediated signal transduction pathways. In essence, we found that c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase ( p38), but not extracellular signal-regulated kinase (ERK), play an important role in the regulation of IL-1 gene expression in RASP-stimulated J774A.1 cells. To the best of our knowledge, this is the first occasion that polysaccharides from R. riparium have been demonstrated to exert immunomodulation properties by the induction of IL-1 within macrophages. Our current results provide support for the possible use of R. riparium as an additive to various food/foodstuff, to modulate the immune response of humans or certain animals.