A comprehensive model of the phototransduction cascade in mouse rod cells
A comprehensive model of the phototransduction cascade in mouse rod cells
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DOI：
10.1039/c3mb70584f
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Bertranpetit, Jaume
中科院分区：
文献类型：
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作者：
Invergo, Brandon M.;Dell'Orco, Daniele;Bertranpetit, Jaume
脊椎动物视觉光转导可能是研究最为深入的G蛋白信号通路。大量可获取的生物化学和电生理数据使得该系统有丰富的数学建模历史。然而，尽管最全面的模型依赖于两栖类的生物化学和电生理数据，但现代研究通常采用哺乳动物物种，特别是小鼠，它们表现出明显更快的信号动态。在这项工作中，我们对先前发表的一个全面的两栖类光转导模型进行了调整，使其能够对小鼠的光反应进行定量准确的模拟。我们展示了该模型预测在广泛的刺激以及多种突变条件下反应的能力。最后，我们利用该模型强调了一种可能与视紫红质和视紫红质激酶之间相互作用相关的未知机制。
Vertebrate visual phototransduction is perhaps the most well-studied G-protein signaling pathway. A wealth of available biochemical and electrophysiological data has resulted in a rich history of mathematical modeling of the system. However, while the most comprehensive models have relied upon amphibian biochemical and electrophysiological data, modern research typically employs mammalian species, particularly mice, which exhibit significantly faster signaling dynamics. In this work, we present an adaptation of a previously published, comprehensive model of amphibian phototransduction that can produce quantitatively accurate simulations of the murine photoresponse. We demonstrate the ability of the model to predict responses to a wide range of stimuli and under a variety of mutant conditions. Finally, we employ the model to highlight a likely unknown mechanism related to the interaction between rhodopsin and rhodopsin kinase.