MiR-214 inhibits snakehead vesiculovirus (SHVV) replication by targeting host GS
MiR-214 inhibits snakehead vesiculovirus (SHVV) replication by targeting host GS
复制标题
MiR-214 通过靶向宿主 GS 抑制蛇头水泡病毒 (SHVV) 复制
DOI：
10.1016/j.fsi.2018.10.028
复制
发表时间：
2019
影响因子：
4.7
通讯作者：
Tu Jiagang
中科院分区：
文献类型：
--
作者：
Zhang Chi;Li Ningqiu;Fu Xiaozhe;Lin Qiang;Lin Li;Tu Jiagang
微小RNA（miRNA）是一种小型非编码RNA，据报道在病毒复制中发挥重要作用。乌鳢水泡病毒（SnakeheadVesiculovirus，SHVV）是从患病的杂交乌鳢中分离到的一种新的弹状病毒，在我国养殖乌鳢中造成了严重的经济损失。我们先前的研究已经揭示了miR-214抑制SHVV复制，但其作用机制尚未完全清楚。在本研究中，糖原合成酶（GS）基因被确定为miR-214的靶基因。miR-214的过表达降低了细胞GS基因的表达。与miR-214的过表达类似，通过siRNA敲低GS抑制SHVV复制。此外，我们发现通过使用miR-214抑制剂降低细胞miR-214水平可以恢复siGS介导的SHVV复制抑制，表明miR-214至少部分地通过靶向GS抑制SHVV。replication.at本研究为进一步了解SHVV致病的分子机制和抗SHVV感染提供了依据。
MicroRNAs (miRNAs) are small noncoding RNAs that have been reported to play important roles in virus re-.plication. Snakehead vesiculovirus (SHVV) is a new rhabdovirus isolated from diseased hybrid snakehead and.has caused heavy economical losses in cultured snakehead fish in China. Our previous study has revealed that.miR-214 inhibited SHVV replication, but the underline mechanism was not completely understood. In this study,.glycogen synthase (GS) gene was identified as a target gene of miR-214. Overexpression of miR-214 reduced.cellular GS gene expression. Knockdown of GS by siRNA, similar to the overexpression of miR-214, inhibited.SHVV replication. Moreover, we found that siGS-mediated inhibition of SHVV replication could be restored by.reducing cellular miR-214 level via using miR-214 inhibitor, indicating that miR-214 inhibited SHVV replication.at least partially via targeting GS. This study provided information for understanding the molecular mechanism.of SHVV pathogenicity and a potential antiviral strategy against SHVV infection.