Spatial and temporal analysis of non-steady elongation of rice leaves
Spatial and temporal analysis of non-steady elongation of rice leaves
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DOI：
10.1111/j.1365-3040.2009.02020.x
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发表时间：
2009-11-01
影响因子：
7.3
通讯作者：
Tardieu, Francois
中科院分区：
文献类型：
--
作者：
Parent, Boris;Conejero, Genevieve;Tardieu, Francois
精确了解细胞分裂和组织扩张的时空分布对于组学研究中适当的叶片取样和植物-环境关系的分析是必不可少的。研究了水稻伸长叶片在整个发育过程中的伸长速率、细胞长度分布、细胞产生速率和生长空间分布。在7个基因型中，叶片伸长速率的模式遵循3个阶段：（1）在叶片出现之前呈指数增长;（2）在叶片出现前后的短阶段（20 ℃下2-4 d），叶片伸长速率稳定;（3）8-10 d的阶段，伸长速率逐渐下降。细胞长度的轮廓沿着叶随时间变化的第一和最后阶段，但在外观周围是时间不变的。我们提出了一种适合于非稳定伸长的解剖测量的基础上，这是成功地测试，通过比较它与针刺方法。它允许分析随着时间的变化，在空间分布的增长，从开始到结束的叶片生长。细胞分裂和组织伸长的叶带长度随时间变化，在叶片出现前后最大分别为21和60 mm。
A precise knowledge of the temporal and spatial distributions of cell division and tissue expansion is essential for appropriate leaf sampling in omics studies and for analyses of plant-environment relations. Elongating leaves of rice were studied during their whole development for elongation rate, distribution of cell length, cell production rate and spatial distribution of growth in the leaf. In seven genotypes, the pattern of leaf elongation rate followed three phases: (1) an exponential increase before leaf appearance; (2) a short phase (2-4 d at 20 degrees C) with a stable leaf elongation rate around leaf appearance; and (3) a phase of 8-10 d with a progressive decrease in elongation rate. The profile of cell length along the leaf changed with time during the first and last phases, but was time invariant around appearance. We propose a method adapted to non-steady elongation based on anatomical measurements, which was successfully tested by comparing it with the pricking method. It allowed analysis of the change with time in the spatial distribution of growth from initiation to end of leaf growth. The length of leaf zones with cell division and tissue elongation varied with time, with maximums of 21 and 60 mm respectively around leaf appearance.