Krapfen/dMyd88 is required for the establishment of dorsoventral pattern in the Drosophila embryo
Krapfen/dMyd88 is required for the establishment of dorsoventral pattern in the Drosophila embryo
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DOI：
10.1016/s0925-4773(02)00410-0
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发表时间：
2003-02-01
影响因子：
2.6
通讯作者：
Moussian, B
中科院分区：
文献类型：
--
作者：
Charatsi, I;Luschnig, S;Moussian, B
在果蝇中，背腹轴是由背侧基因组和仙人掌的作用建立的，它们在遗传上是以线性途径排列的。我们已经确定和特点krapfen（kra）作为背组基因的新成员。kra编码MyD 88的果蝇同源物，MyD 88是一种在哺乳动物IL-1途径中起作用的衔接蛋白。上位性实验表明，kra作用于Toll受体和胞质因子Tube之间。我们发现，有一个直接的相互作用Kra和管推测介导的死亡结构域存在于这两种蛋白质。管进而通过死亡结构域缔合与其下游效应子Pelle相互作用。因此，我们认为，Toll激活后，Kra协会与Pelle和管，在异源三聚体复合物。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
In Drosophila, the dorsoventral axis is set up by the action of the dorsal group of genes and cactus, which have been ordered genetically in a linear pathway. We have identified and characterised krapfen (kra) as a new member of the dorsal-group genes. kra encodes for the Drosophila homologue of MyD88, an adapter protein operating in the mammalian IL-1 pathway. Epistasis experiments reveal that kra acts between the receptor Toll and the cytoplasmic factor Tube. We show that there is a direct interaction between Kra and Tube presumably mediated by the death domains present in both proteins. Tube in turn interacts with its downstream effector Pelle through death domain association. We therefore suggest that upon Toll activation, Kra associates with Pelle and Tube, in an heterotrimeric complex. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.