Magnetic field effects and radical pair mechanisms in enzymes: A reappraisal of the horseradish peroxidase system
Magnetic field effects and radical pair mechanisms in enzymes: A reappraisal of the horseradish peroxidase system
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DOI：
10.1021/ja060463q
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发表时间：
2006-07-05
影响因子：
15
通讯作者：
Woodward, Jonathan R.
中科院分区：
文献类型：
--
作者：
Jones, Alex R.;Scrutton, Nigel S.;Woodward, Jonathan R.
用停流分光光度法重新研究了先前观察到的静态磁场对辣根过氧化物酶(HRP)催化的过氧化氢还原的影响，Taraban等人。(J.Am化学。Soc.1997、119、5768)没有再现最初观察到的效果。对于高达75mT的静电场，没有观察到磁场效应。原始工作中报告的k1和k2的场诱导变化被发现对观察到的418 nm光谱信号的动力学衰减的形状产生了相同和相反的影响，这是由于天然HRP和化合物II的相对吸光度的不同。
Reinvestigation by stopped-flow spectrophotometry of the previously observed influence of a static magnetic field on the horseradish peroxidase (HRP)-catalyzed reduction of hydrogen peroxide by Taraban et al. (J. Am. Chem. Soc.1997,119, 5768) did not reproduce the originally observed effects. No magnetic field effect was observed for static fields of up to 75 mT. Field-induced changes in bothk1andk2reported in the original work were found to produce equal and opposite effects on the shape of the observed kinetic decay of the 418 nm spectroscopic signal as a result of the difference in the relative absorbances of Native HRP and Compound II.