Characterization of the nuclear import signal of herpes simplex virus 1 UL31
Characterization of the nuclear import signal of herpes simplex virus 1 UL31
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单纯疱疹病毒 1 UL31 核输入信号的表征
DOI：
10.1007/s00705-016-2910-z
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发表时间：
2016-09-01
影响因子：
2.7
通讯作者：
Li, Meili
中科院分区：
文献类型：
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作者：
Cai, Mingsheng;Chen, Daixiong;Li, Meili
单纯疱疹病毒1型(HSV-1)UL31蛋白是一种多功能核蛋白，在病毒感染中起重要作用，但对其亚细胞定位信号知之甚少。通过将一系列HSV-1 UL31缺失突变体与增强型黄色荧光蛋白(EYFP)融合，鉴定了一个二分核定位信号(NLS)，并将其定位于氨基酸(AA)1-27(MYDTDPHRRGSRPGPYHGKERRRSRSS)。此外，荧光结果显示，预测的核输出信号(NES)可能是非功能性的，UL31的功能性NES可能需要特定的构象。综上所述，这些结果将为研究UL31在HSV-1感染过程中的生物学功能提供重要信息。
The herpes simplex virus 1 (HSV-1) UL31 protein is a multifunctional nucleoprotein that is important for viral infection; however, little is known concerning its subcellular localization signal. Here, by transfection with a series of HSV-1 UL31 deletion mutants fused to enhanced yellow fluorescent protein (EYFP), a bipartite nuclear localization signal (NLS) was identified and mapped to amino acids (aa) 1 to 27 (MYDTDPHRRGSRPGPYHGKERRRSRSS). Additionally, fluorescence results showed that the predicted nuclear export signal (NES) might be nonfunctional, and the functional NES of UL31 might require a specific conformation. Taken together, these results would provide significant information for the study of the biological function of UL31 during HSV-1 infection.