Black blood gradient echo cine magnetic resonance imaging of the mouse heart
Black blood gradient echo cine magnetic resonance imaging of the mouse heart
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DOI：
10.1002/mrm.20487
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发表时间：
2005-05-01
影响因子：
3.3
通讯作者：
Epstein, FH
中科院分区：
文献类型：
--
作者：
Berr, SS;Roy, RJ;Epstein, FH
在4.7 T扫描仪上实现了小鼠心脏多相心脏MRI的黑色血液梯度回波序列，并与传统的亮血序列进行了比较。使用双反转恢复技术获得黑血。使用亮血和黑血序列对10只小鼠进行成像，另外使用经修改以执行心肌标记的亮血和黑血序列对其中2只小鼠进行成像。由两名独立的观察者对图像进行手动平面测量，以检测心内膜和心外膜边界，随后计算腔室容积和心肌质量。切除的左心室重量用作心肌质量的金标准。Bland-Altman分析表明，与亮血序列相比，使用黑血序列测量心脏容积的观察者间变异性降低（黑血的95%置信区间为-0.89-0.73 mu L，亮血为-1.86-1.28 mu L）。此外，Bland-Altman分析表明，与亮血序列相比，黑血序列提供了更高的心肌质量测量准确性（MRI与体重之间的平均差异为黑血0.9 μ g，亮血-11.2 μ g，P < 0.01）。对于心肌标记，定性评估表明使用黑血序列改善了心肌内膜边界定义。与传统的亮血技术相比，小鼠黑血电影MRI降低了观察者间的变异性，提高了心肌体积和质量测量的准确性。(c)2005 Wiley-Liss，Inc.
A black blood gradient echo sequence for multiphase cardiac MRI of the mouse heart was implemented on a 4.7-T scanner and compared to a conventional bright blood sequence. Black blood was achieved using the double inversion recovery technique. Ten mice were imaged using both the bright and the black blood sequences, and 2 of the mice were additionally imaged using bright and black blood sequences modified to perform myocardial tagging. Manual planimetry of the images was performed by two independent observers to detect the endocardial and epicardial borders and subsequently to compute chamber volumes and myocardial mass. Weight of the excised left ventricle was used as a gold standard for myocardial mass. Bland-Altman analysis demonstrated reduced interobserver variability for the measurement of cardiac volumes using the black blood sequence compared to the bright blood sequence (95% confidence interval was -0.89-0.73 mu L for black blood versus -1.86-1.28 mu L for bright blood). Also, Bland-Altman analysis showed that the black blood sequence provides improved accuracy for the measurement of myocardial mass compared to the bright blood sequence (average difference between MRI versus weight was 0.9 mu g for black blood and -11.2 mu g for bright blood, P < 0.01). For myocardial tagging, qualitative assessment demonstrated improved endocardial border definition using the black blood sequence. Black blood cine MRI in mice provides reduced interobserver variability and improved accuracy for the measurement of myocardial volumes and mass compared to the conventional bright blood technique. (c) 2005 Wiley-Liss, Inc.