Expression of growth differentiation factor 15 is positively correlated with histopathological malignant grade and in vitro cell proliferation in oral squamous cell carcinoma
Expression of growth differentiation factor 15 is positively correlated with histopathological malignant grade and in vitro cell proliferation in oral squamous cell carcinoma
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口腔鳞癌中生长分化因子15的表达与组织病理学恶性程度及体外细胞增殖呈正相关
DOI：
10.1016/j.oraloncology.2008.07.017
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发表时间：
2009-07-01
期刊：
影响因子：
4.8
通讯作者：
Zhang, Zhi-yuan
中科院分区：
文献类型：
--
作者：
Zhang, Lei;Yang, Xiao;Zhang, Zhi-yuan
在此之前，我们建立了一个体外细胞癌变模型的口腔鳞状细胞癌（OSCC）和表达芯片分析表明，基因编码的生长分化因子15（GDF 15）在这个模型中显着上调。在这项研究中，我们证实了GDF15的表达增加在mRNA和蛋白质水平在一组的口腔鳞癌细胞系和临床样本从原发性口腔鳞癌患者。我们还观察到GDF15的表达与疾病的恶性程度呈正相关：GDF15表达水平越高，表明OSCC的恶性程度越高。用针对GDF15的siRNA处理OSCC细胞系（Tca3118）显著抑制细胞增殖和集落形成。基于这些观察，我们得出结论，GDF 15是一个积极的基因的发展和进展，GDF 15可以作为一个额外的标志物，OSCC分化的组织病理学评价。(C)2008爱思唯尔有限公司保留所有权利。
Previously, we established an in vitro cellular carcinogenesis model of oral squamous cell carcinoma (OSCC) and expression microarray analysis showed that the gene encoding growth differentiation factor 15 (GDF15) was significantly upregulated in this model. In this study, we confirmed that expression of GDF15 was increased both at mRNA and protein levels in a panel of OSCC lines and clinical samples from primary OSCC patients. We also observed that expression of GDF15 was positively correlated with the malignancy of the disease: a higher level of GDF15 expression indicates a higher malignant grade of OSCC. Treatment of OSCC cell line (Tca3118) with siRNA against GDF15 significantly inhibited cellular proliferation and colony formation. Based on these observations, we conclude that GDF15 is a positive gene of OSCC development and progression and GDF15 can be used as an additional marker for histopathologic evaluation of OSCC differentiation. (C) 2008 Elsevier Ltd. All rights reserved.