Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction.
Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction.
复制标题
DOI：
10.1021/acs.analchem.6b00403
复制
发表时间：
2016-05
影响因子：
7.4
通讯作者：
Shengjun Yang;Weiping Jiang;Lili Ren;Yaping Yuan;Bin Zhang;Qing Luo;Qianni Guo;L. Bouchard;Maili Liu;Xin Zhou
中科院分区：
文献类型：
--
作者：
Shengjun Yang;Weiping Jiang;Lili Ren;Yaping Yuan;Bin Zhang;Qing Luo;Qianni Guo;L. Bouchard;Maili Liu;Xin Zhou
半胱氨酸（Cys）、同型半胱氨酸（Hcy）和谷胱甘肽（GSH）等生物硫醇在调节生物体的生命功能中起着重要作用。实时了解它们的生物分布可以帮助诊断各种疾病。然而，现有的生物硫醇检测方法是侵入性的并且需要测定。本文报道了一种利用（129）π自旋共振检测生物硫醇的分子生物传感器。（129）氙生物传感器由一个密封氙原子和丙烯酸酯基团的隐晶笼组成。后者作为反应位点通过硫醇加成反应共价键合生物硫醇。该生物传感器能够通过化学位移和平均反应速率将Cys与Hcy和GSH区分开。该传感器可在10 μM的浓度下进行单次扫描检测，并已用于牛血清溶液中生物硫醇的检测。我们的研究结果表明，这种生物传感器是一个很有前途的工具，实时成像的生物硫醇分布。
Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play an important role in regulating the vital functions of living organisms. Knowledge of their biodistribution in real-time could help diagnose a variety of conditions. However, existing methods of biothiol detection are invasive and require assays. Herein we report a molecular biosensor for biothiol detection using the nuclear spin resonance of (129)Xe. The (129)Xe biosensor consists of a cryptophane cage encapsulating a xenon atom and an acrylate group. The latter serves as a reactive site to covalently bond biothiols through a thiol-addition reaction. The biosensor enables discrimination of Cys from Hcy and GSH through the chemical shift and average reaction rate. This biosensor can be detected at a concentration of 10 μM in a single scan and it has been applied to detect biothiols in bovine serum solution. Our results indicate that this biosensor is a promising tool for the real-time imaging of biothiol distributions.