Polymer-based devices for the label-free detection of DNA in solution: low DNA concentrations yield large signals
Polymer-based devices for the label-free detection of DNA in solution: low DNA concentrations yield large signals
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DOI：
10.1007/s00216-014-7867-8
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发表时间：
2014-07-01
影响因子：
4.3
通讯作者：
Serpe, Michael J.
中科院分区：
文献类型：
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作者：
Islam, Molla R.;Serpe, Michael J.
聚(N-异丙基丙烯酰胺-N-(3-氨丙基)甲基丙烯酰胺盐酸盐)微凝胶光学器件(标准具)在单链DNA存在的情况下改变了它们的光学性质。我们假设这是由于带负电的DNA穿透标准具的Au覆盖层，导致带正电的微凝胶的交联和崩塌。我们已经证明，这项技术能够在包含两个和四个碱基对错配序列的溶液中检测目标DNA的微摩尔浓度，而不使用标记。此外，该设备的响应随着DNA浓度的降低而增加，这对于传感策略来说是独一无二的。我们指出，将这种转导机制与DNA扩增策略相结合可能会导致极低的检测限。
Poly (N-isopropylacrylamide-co-N-(3-aminopropyl) methacrylamide hydrochloride) microgel-based optical devices (etalons) have been shown to change their optical properties in the presence of single-stranded DNA. We hypothesize that this is due to the negatively charged DNA penetrating through the Au overlayer of the etalon, resulting in cross-linking and collapse of the positively charged microgels. We have shown that this technology is capable of detecting micromolar concentrations of target DNA in solutions containing two and four base pair mismatch sequences without the use of labels. Furthermore, the device's response increases as the concentration of DNA decreases, which is unique for sensing strategies. We point out that coupling this transduction mechanism to DNA amplification strategies could result in extremely low detection limits.