OXIDATIVE STRESS-INDUCED BY CHRONIC ADMINISTRATION OF SODIUM DICHROMATE [CR(VI)] TO RATS
OXIDATIVE STRESS-INDUCED BY CHRONIC ADMINISTRATION OF SODIUM DICHROMATE [CR(VI)] TO RATS
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DOI：
10.1016/0742-8413(94)00103-h
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发表时间：
1995-03-01
期刊：
影响因子：
--
通讯作者：
STOHS, SJ
中科院分区：
文献类型：
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作者：
BAGCHI, D;HASSOUN, EA;STOHS, SJ
铬主要以价态Cr（III）和Cr（VI）存在于工作场所，最近的研究表明，与Cr（III）相比，重铬酸钠[Cr（VI）]诱导更大的氧化应激，如腹膜巨噬细胞和肝线粒体和微粒体产生活性氧所示，急性口服Cr（III）和Cr（VI）后尿脂质代谢产物和肝DNA单链断裂（SSB）的排泄增加，因此，我们研究了重铬酸钠二水合物[Cr（VI）; 10 mg（33.56 μ mol）/kg/天]对肝线粒体和微粒体脂质过氧化，增加尿脂质代谢产物的排泄，包括丙二醛（MDA），甲醛（FA），在给药约45天时观察到肝脂质过氧化和DNA损伤的最大增加，MDA、FA、ACT、ACON和PROP的尿排泄的最大增加分别为3.2-、2.6-、4.1-和4.1-。结果清楚地表明，慢性重铬酸钠给药诱导氧化应激，导致组织损伤效应，这可能有助于六价铬的毒性和致癌性。
Chromium occurs in the workplace primarily in the valence forms Cr(III) and Cr(VI), Recent studies have demonstrated that sodium dichromate [Cr(VI)] induces greater oxidative stress as compared with Cr(III), as indicated by the production of reactive oxygen species by peritoneal macrophages and hepatic mitochondria and microsomes, and enhanced excretion of urinary lipid metabolites and hepatic DNA-single strand breaks (SSB) following acute oral administration of Cr(III) and Cr(VI), We have therefore examined the chronic effects of sodium dichromate dihydrate [Cr(VI); 10 mg (33.56 mu mol)/kg/day] on hepatic mitochondrial and microsomal lipid peroxidation, enhanced excretion of urinary lipid metabolites including malondialdehyde (MDA), formaldehyde (FA), acetaldehyde (ACT), acetone (ACON) and propionaldehyde (PROP), and hepatic DNA damage over a period of 90 days, The maximal increases in hepatic lipid peroxidation and DNA damage were observed at approximately 45 days of treatment, Maximum increases in the urinary excretion of MDA, FA, ACT, ACON and PROP were 3.2-, 2.6-, 4.1-, 3.3- and 2.1-fold, respectively, while a 5.2-fold increase in DNA-SSB was observed, The results clearly indicate that chronic sodium dichromate administration induces oxidative stress resulting in tissue damaging effects which may contribute to the toxicity and carcinogenicity of hexavalent chromium.