Phylogeny and Evolution of Apusomonadida (Protozoa: Apusozoa): New Genera and Species
Phylogeny and Evolution of Apusomonadida (Protozoa: Apusozoa): New Genera and Species
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DOI：
10.1016/j.protis.2010.04.002
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发表时间：
2010-10-01
期刊：
影响因子：
2.5
通讯作者：
Chao, Ema E.
中科院分区：
文献类型：
--
作者：
Cavalier-Smith, Thomas;Chao, Ema E.
Apusomonadida（Apusomonas; Amastigomonas）是正在研究的滑行鞭毛虫。我们将无鞭毛单胞菌分为五个属，其中三个是新的属：Poplum属; Manchomonas属; Multimonas属。显微镜和18 S rDNA序列建立了三个新的海洋物种（大Poplomagna; P. capensis; Multimonas media）和一个新的包囊形成的非海洋物种从常春藤叶表面（Thecamonas oxoniensis）。我们认为土壤和淡水Amastigomonas debruynei，caudata和borokensis一般不同于海洋Thecamonas。我们建立了新的组合Multimonas marina（以前的Cercomonas或Amastigomonas）。我们研究了DIC显微镜和18 S rDNA测序三个菌株显微镜下无法区分海洋Thecamonas trahens和认为，海洋菌株几乎相同的序列和外观（可见主要是首字母纤毛）以前被误认为是上午。debruynei。我们认为，'Amastigomonas sp. ATCC 50062为T. trahens。我们包括T. aff. trahens，P. capensis and magna; P. capensis，Thecamonas，and Manchomonas（=Amastigomonas）bermudensis comb. n.允许对无单胞菌进化进行新的功能解释。在18 SrDNA树上，Apusomonas和Manchomonas形成一个强大的分支（Apusomonadinae），而Thecomonastrahens、T. oxoniensis、Multimonas和Poxoniensis的分支深但不稳定。Apusomonadida和Planomonas是后鞭毛动物的弱姐妹。(c)2010年Elsevier GmbH。All rights reserved.
Apusomonadida (Apusomonas; Amastigomonas) are under studied gliding zooflagellates. We divide Amastigomonas into five genera, three new: Podomonas; Manchomonas; Multimonas. Microscopy and 18S rDNA sequences establish three new marine species(Podomonas magna; P. capensis; Multimonas media) and a new cyst-forming non-marine species from the surface of ivy leaves (Thecamonas oxoniensis). We consider the soil and freshwater Amastigomonas debruynei, caudata, and borokensis generically distinct from marine Thecamonas. We establish the new combination Multimonas marina (formerly Cercomonas or Amastigomonas). We studied by DIC microscopy and 18S rDNA sequencing three strains microscopically indistinguishable from marine Thecamonas trahens and argue that marine strains of almost identical sequence and appearance (visible largely acronematic cilia) were previously misidentified as Am. debruynei. We argue that 'Amastigomonas sp.' ATCC50062, whose 18S rRNA was sequenced previously and whose complete genome is being sequenced, is T. trahens. We include electron micrographs of T. aff. trahens, P. capensis and magna; ultrastructural cytoskeletal differences between P. capensis, Thecamonas, and Manchomonas (=Amastigomonas) bermudensis comb. n. allow novel functional interpretations of apusomonad evolution. On 18Sr DNA trees Apusomonas and Manchomonas form a robust clade (Apusomonadinae), but Thecomonas trahens, T. oxoniensis, Multimonas, and Podomonas all branch deeply but unstably. Apusomonadida and Planomonas are weakly sister to opisthokonts. (c) 2010 Elsevier GmbH. All rights reserved.