Limited Susceptibility and Lack of Systemic Infection by an H3N2 Swine Influenza Virus in Intranasally Inoculated Chickens
Limited Susceptibility and Lack of Systemic Infection by an H3N2 Swine Influenza Virus in Intranasally Inoculated Chickens
复制标题
DOI：
10.1637/8210-011408-resnote.1
复制
发表时间：
2008-09-01
期刊：
影响因子：
1.4
通讯作者：
Swayne, David E.
中科院分区：
文献类型：
--
作者：
Thomas, Colleen;Manin, Timofey B.;Swayne, David E.
将猪流感病毒(SIV)A/SIVE/NC/307408/04(H3N2)(NC/04SIV)经鼻接种鸡，以确定北美SIV对鸡的传染性，以及鸡肉作为SIV传播媒介的可能性。对白色来航鸡(WL)进行了初步的病原学分型和传染性试验，并对白普利茅斯罗克(WPR)肉鸡进行了更全面的鼻腔致病研究。接种NC/04SIV的WL和WPR鸡均未出现临床症状。血清学试验表明，该病毒能够感染经鼻接种的WL和WPR鸡，但抗体效价较低，表明复制效率低下。接种NC/04 SIV的WL鸡部分病毒水平较低，主要来自消化道，但在NC/04 SIV接种的WPR鸡中未检测到病毒脱落。综合致病机理研究表明，该病毒不会引起WPR鸡的全身性感染，用NC/04SIV接种WPR的胸肉和大腿肉喂养WL鸡不会传播NC/04SIV。
Chickens were intranasally inoculated with the swine influenza virus (SIV) A/swine/NC/307408/04 (H3N2) (NC/04 SIV) to determine the infectivity of a North American SIV for chickens, as well as the possibility of chicken meat serving as a transmission vehicle for SIV. White leghorn (WL) layer-type chickens were used for initial pathotyping and infectivity tests, and a more comprehensive intranasal pathogenesis study was done with white Plymouth rock (WPR) broiler-type chickens. None of the NC/04 SIV-inoculated WL or WPR chickens displayed clinical signs. Serologic tests showed that the virus was able to infect both intranasally inoculated WL and WPR chickens, but the antibody titers were low, suggesting inefficient replication. Some of the NC/04 SIV-inoculated WL chickens shed low levels of virus, mostly from the alimentary tract, but viral shedding was not detected in NC/04 SIV-inoculated WPR chickens. The comprehensive pathogenesis study demonstrated that the virus did not cause systemic infections in WPR chickens, and feeding breast and thigh meat from the NC/04 SIV-inoculated WPR to WL chickens did not transmit NC/04 SIV.