First report of Turnip yellows virus in Brazil
First report of Turnip yellows virus in Brazil
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巴西首次报告芜菁黄化病毒
DOI：
10.1002/ndr2.12045
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发表时间：
2021
影响因子：
--
通讯作者：
Greer S
中科院分区：
文献类型：
--
作者：
Greer S
甜菜西部黄化病毒（Beetwesternyellowsvirus，BWYV）具有单链、正义RNA基因组。它是韧皮部限制和蚜虫传播的持久性（循环，非繁殖）的方式。该病毒对巴西芸苔属植物的生产构成严重威胁，因为它已显示在蔬菜芸苔属植物中导致高达65%的产量损失（沃尔什等人，2011年）。在20世纪70年代和80年代，巴西报道了野生萝卜植物的感染，下部叶子出现黄化症状，可能是由BWYV引起的（Kitajima，2020），但病原学尚未得到证实。2017年5月，一棵卷心菜（Brassica oleracea var.在巴西巴拉那州阿劳卡里亚市的一片商业田地中发现了叶子变红的Capitata）植物（图1）。TuYV的症状叶组织的酶联免疫吸附测定（Hackenberg等人，2019）给出了明确的积极结果。通过RNA提取和特异引物RT-PCR检测，未检测到芜菁花叶病毒（TuMV）和科尔潜伏病毒（CoLV）的侵染。花椰菜花叶病毒（CaMV）经DNA提取和PCR检测均未检出。机械接种的症状叶组织的指示植物的范围内未能检测到任何病毒的存在。2021年7月，从巴西圣保罗州主要蔬菜芸苔属作物产区伊比乌纳市的两个商业田地（图2）收集了六株在老叶中显示变红症状的卷心菜植物的叶子。症状植株的发生率为c。百分之一通过RT-PCR测试的所有有症状的植物，
Beet western yellows virus, BWYV) has a single-stranded, positive-sense RNA genome. It is phloem-limited and aphid-transmitted in a persistent (circulative, non-propagative) manner. The virus represents a serious threat to Brazilian brassica production as it has been shown to cause yield losses of up to 65% in vegetable brassicas (Walsh et al., 2011). In the 1970’s and 1980’s, infections of wild radish plants with yellowing symptoms in lower leaves were reported in Brazil, probably caused by BWYV (Kitajima, 2020) though the aetiology was not confirmed. In May 2017, a cabbage (Brassica oleracea var. capitata) plant with reddening of leaves was discovered in a commercial field in the municipality of Araucária, Paraná State, Brazil (Fig. 1). Enzyme-linked immunosorbent assay of the symptomatic leaf tissue for TuYV (Hackenberg et al., 2019) gave a clear positive result. No infection by Turnip mosaic virus (TuMV) or Cole latent virus (CoLV) was detected following RNA extraction and RT-PCR with specific primers. Cauliflower mosaic virus (CaMV) was not detected following DNA extraction and PCR. Mechanical inoculation of symptomatic leaf tissue to a range of indicator plants failed to detect the presence of any virus. In July 2021, leaves from six cabbage plants showing reddening symptoms in older leaves were collected from two commercial fields in the municipality of Ibiúna (Fig. 2), in the main vegetable brassicaproducing region in São Paulo State, Brazil. The incidence of symptomatic plants was c. 1%. All symptomatic plants tested by RT-PCR with