Pulsatile growth hormone secretion in normal man during a continuous 24-hour infusion of human growth hormone releasing factor (1-40). Evidence for intermittent somatostatin secretion.
Pulsatile growth hormone secretion in normal man during a continuous 24-hour infusion of human growth hormone releasing factor (1-40). Evidence for intermittent somatostatin secretion.
复制标题
正常人在连续 24 小时输注人生长激素释放因子 (1-40) 期间出现脉动生长激素分泌。
DOI：
10.1172/jci111864
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Thorner,MO
中科院分区：
文献类型：
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作者：
Vance,ML;Kaiser,DL;Evans,WS;Furlanetto,R;Vale,W;Rivier,J;Thorner,MO
对1例异位分泌生长激素释放因子(GRF)的患者和持续输注人生长激素释放因子(1-40)-羟基(hGRF-40)的正常人生长激素(GH)分泌模式进行了研究。在异位GRF分泌的患者中，尽管免疫反应性GRF水平持续升高，但GH的分泌是脉动的。为了确定正常受试者是否维持脉动性GH分泌，我们给6名健康青年男性注射赋形剂或hGRF-40，每分钟2 ng/kg，持续24小时，并在输注23.5h后，静脉推注超过最大剂量的hGRF-40，3.3微克/公斤。HGRF-40输注组的GH分泌高于赋形剂输注组，并且在整个hGRF-40输注过程中，GH的分泌始终保持有搏动性。在赋形剂输注的23.5h内，GH总分泌量(微克；平均+/-扫描电子显微镜)为634+/-151，而输注hGRF-40时为1,576+/-284(P=0.042)。静脉推注hGRF-40后的GH反应大于推注后的GH反应，推注后和推注后40d的GH分泌量分别为877+/-170和386+/-125微克(P=0.015)。在两个研究日的25.5小时内，GH的总分泌量没有差异，在车辆和hGRF-40天分别分泌了1,504+/-260和1,952+/-383微克(P=0.36)。在hGRF-40输注期间，不仅保持了GH的脉动分泌，而且自然产生的GH脉冲也得到了增强，这与促性腺激素释放激素对促性腺激素分泌的影响形成了对比。我们认为GH脉冲是GRF分泌的结果，而GRF分泌与生长抑素分泌的减少或停止有关。
Growth hormone (GH) secretory patterns were studied in a patient with ectopic growth hormone releasing factor (GRF) secretion and in normal men given continuous infusions of human growth hormone releasing factor (1-40)-OH (hGRF-40). In the patient with ectopic GRF secretion, GH secretion was pulsatile despite continuously elevated immunoreactive GRF levels. To determine if pulsatile GH secretion is maintained in normal subjects, we administered to six healthy young men vehicle or hGRF-40, 2 ng/kg per min, for 24 h and gave a supramaximal intravenous bolus dose of hGRF-40, 3.3 micrograms/kg, after 23.5 h of infusion. hGRF-40 infusion resulted in greater GH secretion than did vehicle infusion and pulsatile GH secretion was maintained throughout hGRF-40 infusion. During the 23.5 h of vehicle infusion, total GH secretion (microgram; mean +/- SEM) was 634 +/- 151 compared with 1,576 +/- 284 during hGRF-40 infusion (P = 0.042). The GH response to the intravenous bolus of hGRF-40 was greater after vehicle infusion than after hGRF-40 infusion; 877 +/- 170 and 386 +/- 125 micrograms of GH was secreted after the bolus on vehicle and hGRF-40 days, respectively (P = 0.015). The total amount of GH secreted during the 25.5 h of the two study days was not different; 1,504 +/- 260 and 1,952 +/- 383 micrograms were secreted during vehicle and hGRF-40 days, respectively (P = 0.36). Not only was pulsatile GH secretion maintained during hGRF-40 infusion, but there was augmentation of naturally occurring GH pulses, which is in contrast to the effect of gonadotropin-releasing hormone on gonadotropin secretion. We suggest that GH pulses are a result of GRF secretion that is associated with a diminution or withdrawal of somatostatin secretion.