The nature and magnitude of in vivo 5-hydroxyindoleacetic acid output from 5-hydroxytryptamine terminals is related to specific regions of the suprachiasmatic nucleus.
The nature and magnitude of in vivo 5-hydroxyindoleacetic acid output from 5-hydroxytryptamine terminals is related to specific regions of the suprachiasmatic nucleus.
复制标题
体内 5-羟基色胺末端输出 5-羟基吲哚乙酸的性质和大小与视交叉上核的特定区域有关。
DOI：
10.1159/000124857
复制
发表时间：
1987
影响因子：
4.1
通讯作者：
Meyer,DC
中科院分区：
文献类型：
--
作者：
Ramirez,AD;Ramirez,VD;Meyer,DC
8只骑自行车的雌性大鼠在视交叉上核(SCN)区域植入推拉套管，恢复时间为7-10天。在这些自由行为的大鼠中，SCN的灌流持续5-6h的光期和主观暗点。5-羟基吲哚乙酸(5-HIAA)的释放量为10~350pg/min。值得注意的是，5-HIAA输出的幅度和特征高度依赖于位置，因为吻端插管的幅度变化很大，初始平均值为40pg 5-HIAA/min，向暗相增加，平均峰值为195pg 5-HIAA/min。尾管放置显示低幅度、高频率的基础型5-HIAA释放，在接近暗期(约5pg/min)时不会增加。5-羟色氨酸输注使5-HIAA的基础释放显着增加，证实了灌注区的生化活性。这些结果表明，在体测得5-羟色胺(5-HT)终末在SCN区域的5-HIAA可以反映自由活动大鼠SCN特定区域内5-羟色胺能终末的离散功能活动。此外，这些5-羟色胺终末的生化活性和大鼠SCN在5-羟色胺活性上的显著差异变化强调了该模型系统在研究自由行为动物的神经内分泌事件方面的生理学意义。
Eight cycling female rats were implanted with push-pull cannulae over the region of the suprachiasmatic nuclei (SCN) and allowed 7–10 days for recovery. Perfusion of the SCN continued in these freely behaving rats for 5–6 h of the light period and the subjective scotophase. The release of 5-hydroxyindoleacetic acid (5-HIAA) ranged from 10 to 350 pg 5-HIAA/min. Significantly, the amplitude and characteristics of the output of 5-HIAA were highly location dependent in that rostral cannulae placements revealed high amplitude changes with initial mean values of 40 pg 5-HIAA/min, which increased toward the dark phase to mean peak values of 195 pg 5-HIAA/min. Caudal cannulae placements revealed a low amplitude, high frequency, basal type of 5-HIAA release which did not increase toward the dark period (approximately 5 pg/min). 5-Hydroxytryptophan infusion resulted in a significant marked increase in the basal release of 5-HIAA confirming the biochemical viability of the area undergoing perfusion. These results suggest that the in vivo measurement of 5-HIAA from 5-hydroxytryptamine (5-HT) terminals in the region of the SCN could reflect discrete functional activity of serotonergic terminals within specific regions of the SCN in a freely behaving rat. Furthermore, the biochemical viability of these 5-HT terminals and the ability of the rat’s SCN to exhibit marked differential changes in 5-HT activity emphasizes the physiological relevance of this model system to study neuroendocrine events in freely behaving animals.