Production of biogenic manganese oxides by anamorphic ascomycete fungi isolated from streambed pebbles
Production of biogenic manganese oxides by anamorphic ascomycete fungi isolated from streambed pebbles
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DOI：
10.1080/01490450500533809
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发表时间：
2006-03-01
影响因子：
2.3
通讯作者：
Iwahori, K
中科院分区：
文献类型：
--
作者：
Miyata, N;Maruo, K;Iwahori, K
我们的特点是生产生物锰氧化物的四个变形子囊真菌分离出河床卵石锰氧化物涂层。基于18 S rRNA基因序列，一个菌株与Xylariales的成员有关，其他三个菌株在Pleosporales的不同谱系内。这些菌株氧化锰（II）存款锰氧化物时，他们的增长接近稳定期。真菌Mn氧化物显示出典型的差结晶水钠石（δ-MnO 2）的X射线衍射图案，并且X射线吸收近边结构光谱证实Mn相主要由Mn（IV）组成。Mn（II）氧化在四个菌株进行酶促。Mn（II）氧化蛋白被叠氮化合物和邻菲咯啉抑制，并且该蛋白还氧化典型的lac-case底物，包括2，2 '-azinobis（3-ethylbenzothiazoline-6-sulfonic acid）（ABTS），显示漆酶或漆酶样金属酶的作用。生物锰氧化物的矿物学特征，和漆酶样酶的参与，是根据我们以前的结果与一个肉座菌子囊菌。总之，不同的子囊菌可能会使用这种常见的酶系统，以产生固体锰相类似的delta-MnO 2。
We characterized the production of biogenic Mn oxides by four anamorphic ascomycete fungi isolated from streambed pebbles with Mn oxide coatings. Based on the 18S rRNA gene sequences, one strain was related to members of the order Xylariales and the other three were within distinct lineages of the Pleosporales. These strains oxidized Mn(II) to deposit Mn oxides when their growth approached the stationary phase. The fungal Mn oxides showed X-ray diffraction patterns typical of poorly crystalline vernadite (delta-MnO2), and X- ray absorption near-edge structure spectroscopy confirmed that the Mn phases consisted predominantly of Mn(IV). Mn(II) oxidation in the four strains proceeded enzymatically. The Mn(II)-oxidizing proteins were inhibited by azide and o-phenanthroline, and the proteins also oxidized typical lac-case substrates including 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), showing the role of laccase or a laccase-like metalloenzyme. The mineralogical traits of the biogenic Mn oxides, and the participation of laccase-like enzymes, are in accordance with our previous results obtained with one Hypocreales ascomycete. In conclusion, phylogenetically diverse ascomycetes may use this common enzymatic system to produce solid Mn phases similar to delta-MnO2.