Protein-resistant coatings for glass and metal oxide surfaces derived from oligo(ethylene glycol)-terminated alkyltrichlorosilanes
Protein-resistant coatings for glass and metal oxide surfaces derived from oligo(ethylene glycol)-terminated alkyltrichlorosilanes
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DOI：
10.1016/s0142-9612(98)00044-1
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发表时间：
1998-09-01
期刊：
影响因子：
14
通讯作者：
Laibinis, PE
中科院分区：
文献类型：
--
作者：
Lee, SW;Laibinis, PE
本文介绍了低聚乙二醇封端的烷基三氯硅烷Cl_3Si（CH_2）（11）（OCH_2CH_2）（n）OCH_3（n = 2，3）的制备及其在氧化物表面自组装单分子膜形成中的应用。三氯硅烷从溶液中的吸附产生了厚度为2-3 nm的致密堆积的取向单层膜。三氯甲硅烷基将分子锚定在表面，所得膜在其表面暴露乙二醇单元，如其适度亲水性（θ（a）（H2O）约为68度）所示。该膜具有与其他烷基硅氧烷涂层相似的稳定性。这些低聚（乙二醇）封端的硅烷试剂产生的薄膜显着表现出对溶液中蛋白质非特异性吸附的抵抗力，比由十八烷基三氯硅烷制备的薄膜更好。与胰岛素，溶菌酶，白蛋白，和己糖激酶，没有观察到吸附与低聚（乙二醇）-硅氧烷涂层，而蛋白质膜的约单层表面上形成的十八烷基三氯硅烷处理。对于纤维蛋白原，两种涂层都不可能具有完全的抵抗力;然而，低聚（乙二醇）-硅氧烷涂层对非特异性吸附具有更大的抵抗力。低聚（乙二醇）-硅氧烷涂层提供了优于现有系统的性能优势，并且可以容易地在目前使用硅烷试剂来产生疏水性“惰性”表面的方案中提供直接和上级替代。(C)1998爱思唯尔科技有限公司版权所有。
This paper describes the preparation of oligo(ethylene glycol)-terminated alkyltrichlorosilanes, Cl3Si(CH2)(11)(OCH2CH2)(n)OCH3 (n = 2, 3), and their use in the formation of self-assembled monolayers on an oxide surface. The adsorption of the trichlorosilanes from solution produces densely packed, oriented monolayer films that are 2-3 nm in thickness. The trichlorosilyl group anchors the molecules to the surface, and the resulting film exposes the ethylene glycol units at its surface, as noted by its moderate hydrophilicity (theta(a)(H2O) approximate to 68 degrees). The films are robust with stabilities similar to those of other alkylsiloxane coatings. These oligo(ethylene glycol)-terminated silane reagents produce films that notably exhibit resistances against the non-specific adsorption of proteins from solution that are better than for films prepared from octadecyltrichlorosilane. With insulin, lysozyme, albumin, and hexokinase, no adsorption was observed with the oligo(ethylene glycol)-siloxane coatings whereas protein films of approximately a monolayer formed on surfaces-treated with octadecyltrichlorosilane. With fibrinogen, complete resistance was not possible with either coating; however, the oligo(ethylene glycol)-siloxane coatings exhibited greater resistance against non-specific adsorption. The oligo(ethylene glycol)-siloxane coatings offer performance advantages over available systems and could easily provide a direct and superior replacement in protocols that presently use silane reagents to generate hydrophobic,'inert' surfaces. (C) 1998 Elsevier Science Ltd. All rights reserved.