Isolation, Purification, and Culture of Primary Murine Sensory Neurons.
Isolation, Purification, and Culture of Primary Murine Sensory Neurons.
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DOI：
10.1007/978-1-4939-7237-1_15
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Leib DA
中科院分区：
文献类型：
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作者：
Katzenell S;Cabrera JR;North BJ;Leib DA
培养的原代神经元对于神经元解剖学、细胞生物学和生理学的研究具有非凡的价值。虽然神经元细胞系的使用具有方便性和实用性，但由于它们的有丝分裂性质，经常存在警告。本文介绍了用于研究单纯疱疹病毒溶解性和潜伏性感染的成年小鼠感觉神经元的制备、纯化和培养的详细方法。虽然病毒学是我们实验室的应用，但这些培养物对神经生物学家和细胞生物学家也有广泛的用途。虽然这些原代培养物具有很高的信息量，但对许多研究人员来说，这种方法具有挑战性。通过出版这个非常详细的协议，这是我们的希望，这种培养系统的使用可以在该领域的推广，让我们更快地了解嗜神经病毒感染的进展。
Cultured primary neurons have been of extraordinary value for the study of neuronal anatomy, cell biology, and physiology. While use of neuronal cell lines has ease and utility, there are often caveats that arise due to their mitotic nature. This methods article presents detailed methodology for the preparation, purification, and culture of adult murine sensory neurons for the study of herpes simplex virus lytic and latent infections. While virology is the application for our laboratory, these cultures also have broad utility for neurobiologists and cell biologists. While these primary cultures have been highly informative, the methodology is challenging to many investigators. Through publication of this highly detailed protocol, it is our hope that the use of this culture system can spread in the field to allow more rapid progress in furthering our understanding of neurotropic virus infection.