Single Gold@Silver Nanoprobes for Real-Time Tracing the Entire Autophagy Process at Single-Cell Level
Single Gold@Silver Nanoprobes for Real-Time Tracing the Entire Autophagy Process at Single-Cell Level
复制标题
单金@银纳米探针用于在单细胞水平上实时追踪整个自噬过程
DOI：
10.1021/ja5112628
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发表时间：
2015-02-11
影响因子：
15
通讯作者：
Zhu, Jun-Jie
中科院分区：
文献类型：
--
作者：
Chen, Zixuan;Li, Jingjing;Zhu, Jun-Jie
本文描述了一种用于在单细胞水平上实时监测整个自噬过程的多重修饰的核壳金@银纳米探针。自噬对于理解人类病理机制、开发新药和探索控制自噬的方法至关重要。自噬研究的一个主要挑战在于实时监测。一种解决方案可能来自于实时检测超氧阴离子自由基(O-2(中心点-))，因为它是自噬的主要调节因子。在这项工作中，我们提出的纳米探针被O-2(中心点-)刻蚀，并在等离子体共振散射光谱中给出了显著的波长变化。实验和模拟结果都表明，波长变化率与O-2(中心点)水平有很好的相关性。这一反应使其能够应用于自噬过程中O-2(中心点-)的实时原位定量。更重要的是，引入了中继式探针操作，自始至终成功地追踪到了两种类型的O-2(中心点)调节自噬过程，并提出了可能的机制。
This article describes a multimodified coreshell gold@silver nanoprobe for real-time monitoring the entire autophagy process at single-cell level. Autophagy is vital for understanding the mechanisms of human pathologies, developing novel drugs, and exploring approaches for autophagy controlling. A major challenge for autophagy study lies in real-time monitoring. One solution might come from real-time detection of in situ superoxide radicals (O-2(center dot-)), because it is the main regulator of autophagy. In this work, our proposed nanoprobes were etched by O-2(center dot-) and gave a notable wavelength change in the plasmon resonance scattering spectra. Both the experimental and simulated results suggested the wavelength change rate correlated well with O-2(center dot-) level. This response enabled its application in real-time in situ quantification of O-2(center dot-) during autophagy course. More importantly, with the introduction of relay probe operation, two types of O-2(center dot-) regulating autophagy processes were successfully traced from the beginning to the end, and the possible mechanism was also proposed.