5-HT2C-like receptors in the brain of Xenopus laevis initiate sex-typical fictive vocalizations.
5-HT2C-like receptors in the brain of Xenopus laevis initiate sex-typical fictive vocalizations.
复制标题
非洲爪蟾大脑中的 5-HT2C 样受体会启动典型性别的虚构发声。
DOI：
10.1152/jn.90469.2008
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发表时间：
2009
影响因子：
2.5
通讯作者：
Yamaguchi,A
中科院分区：
文献类型：
--
作者：
Yu,HJ;Yamaguchi,A
雄性和雌性非洲爪蟾（Xenopus laevis）的发声是由脑干中枢模式发生器产生的。5-羟色胺（5-HT）可能是重要的发声启动，因为，当应用在体外，性典型的虚构发声诱发从孤立的大脑。为了探索发声起始的机制，我们用全脑虚拟标本鉴定了5-羟色胺受体介导发声激活的类型。结果表明，5-HT 2C样受体是重要的激活虚构的发声在性别。5-HT_（2C）受体激动剂可引起雌性动物的虚构发声，5-HT_（2C）受体拮抗剂可阻断5-HT诱导的虚构发声，而5-HT_（2A）和5-HT_（2B）受体激动剂和拮抗剂不能引起或阻断5-HT诱导的虚构发声。结果表明，5-羟色胺启动虚构的发声结合5-HT 2C样受体位于内或上游的发声中枢模式发生器在两种性别。我们的结论是，声音启动的基本机制是共享的性别，尽管男性和女性之间的实际发声的差异。因此，性别典型的发声，最有可能来自不同群体的5-HT 2C受体表达细胞的激活或来自下游模式产生神经元的差异激活。
Vocalizations of male and female African clawed frogs (Xenopus laevis) are generated by brain stem central pattern generators. Serotonin (5-HT) is likely important for vocal initiation because, when applied in vitro, sex-typical fictive vocalizations are evoked from isolated brains. To explore the mechanisms underlying vocal initiation, we identified the types of serotonin receptors mediating vocal activation pharmacologically using a whole brain, fictive preparation. The results showed that 5-HT2C–like receptors are important for activation of fictive vocalizations in the sexes. 5-HT2Creceptor agonists elicited fictive vocalizations, and 5-HT2Creceptor antagonists blocked 5-HT–induced fictive vocalizations, whereas agonists and antagonists of 5-HT2Aand 5-HT2Breceptors failed to initiate or block 5-HT–induced fictive vocalizations in the sexes. The results indicate that serotonin initiates fictive vocalizations by binding to 5-HT2C–like receptors located either within or upstream of the vocal central pattern generator in both sexes. We conclude that the basic mechanism of vocal initiation is shared by the sexes despite the differences in the actual vocalizations between males and females. Sex-typical vocalizations, therefore, most likely arise from activation of different populations of 5-HT2Creceptor expressing cells or from differential activation of downstream pattern generating neurons.