Precisely Programmed and Robust 2D Streptavidin Nanoarrays by Using Periodical Nanometer-Scale Wells Embedded in DNA Origami Assembly
Precisely Programmed and Robust 2D Streptavidin Nanoarrays by Using Periodical Nanometer-Scale Wells Embedded in DNA Origami Assembly
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DOI：
10.1002/cbic.200900229
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发表时间：
2009-07-20
期刊：
影响因子：
3.2
通讯作者：
Komiyama, Makoto
中科院分区：
文献类型：
--
作者：
Kuzuya, Akinori;Kimura, Mayumi;Komiyama, Makoto
设计并构建了一种新型的具有周期性纳米级威尔斯孔的穿孔DNA“折纸”组装体。通过在每个孔的两个边缘连接两种生物素，每个6.8 x 12 x 2.0 nm孔中仅尺寸选择性捕获一种链霉亲和素（SA）四聚体（d=5 nm）;这允许形成28纳米周期的SA单个分子纳米阵列。SA捕获的位置可以通过在纳米阵列孔中放置生物素来完全控制。此外，通过将SA四聚体选择性定位在这种穿孔折纸基序的复合物中的任何期望的威尔斯孔中，也可以构建单个SA四聚体的2D纳米阵列。在相同分子上直接比较通过该固定策略（DNA威尔斯孔和两个生物素接头）捕获的SA的稳定性与通过不使用威尔斯孔或两个接头的其它可能策略捕获的SA的稳定性。通过这种方式，明确确立了这种固定方法的稳健性。
A new punched DNA origami assembly with periodic nanometer-scale wells has been successfully designed and constructed. Through the attachment of two biotins at the two edges of each well, just one streptavidin (SA) tetramer (d=5 nm) was size-selectively captured in each 6.8 x 12 x 2.0 nm well; this allowed formation of a 28 nm-period SA nanoarray of individual molecules. The position of SA capture can be fully controlled by placement of biotins in the nanoarray well. Moreover, construction of a 2D nanoarray of individual SA tetramers through selective positioning of SA tetramers in any desired wells in a complex of such punched origami motifs is also possible. The stability of the SA captured by this fixation strategy (DNA wells and two biotin linkers) was directly compared on the same molecule with the stability of SA captured with other possible strategies that do not employ wells or two linkers. In this way, the robustness of this means of fixation was clearly established.