Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization of Chondroitin Sulfate Proteoglycans.
Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization of Chondroitin Sulfate Proteoglycans.
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DOI：
10.1007/978-1-0716-2946-8_2
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发表时间：
2023-01-01
期刊：
影响因子：
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通讯作者：
Kitagawa, Hiroshi
中科院分区：
文献类型：
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作者：
Mikami, Tadahisa;Kitagawa, Hiroshi
硫酸软骨素蛋白聚糖 (CSPG) 是聚阴离子细胞外/细胞周基质大分子，几乎包围所有细胞类型，并创造微环境生态位来支持各种细胞事件。一般来说，CSPG 的多功能特性归因于 CS 糖胺聚糖 (GAG) 部分的结构差异。由于 CSPG 的 GAG 链表达谱会随着发育阶段、衰老和疾病进展而变化，因此 GAG 链的表征对于了解 CSPG 的功能作用至关重要。本章介绍了生物样品中基于 GAG 部分的 CSPG 免疫化学检测以及 CS 二糖成分分析的基本方案。
Chondroitin sulfate proteoglycans (CSPGs) are polyanionic extra/pericellular matrix macromolecules that surround almost all cell types and create microenvironmental niches to support miscellaneous cellular events. In general, the multifunctional properties of CSPGs are attributable to the structural divergence of the CS glycosaminoglycan (GAG) moieties. Because the expression profiles of the GAG chains of CSPGs change with developmental stage, aging, and disease progression, characterization of the GAG chains is essential to understand the functional roles of CSPGs. This chapter describes the basic protocols for GAG moiety-based immunochemical detection of CSPGs in biological samples in conjunction with CS disaccharide composition analysis.