CaWRKY28 Cys249 is Required for Interaction with CaWRKY40 in the Regulation of Pepper Immunity to Ralstonia solanacearum
CaWRKY28 Cys249 is Required for Interaction with CaWRKY40 in the Regulation of Pepper Immunity to Ralstonia solanacearum
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CaWRKY28 Cys249 是与 CaWRKY40 相互作用调节辣椒对青枯菌的免疫所必需的
DOI：
10.1094/mpmi-12-20-0361-r
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发表时间：
2021-07-01
影响因子：
3.5
通讯作者：
He，Shuilin
中科院分区：
文献类型：
--
作者：
Yang，Sheng;Zhang，Yangwen;He，Shuilin
WRKY转录因子与植物对病原体的反应有关，但WRKY介导的网络如何组织和运作以产生适当的转录产物仍不清楚。在这里，我们确定了一个成员的WRKY家族辣椒（辣椒），CaWRKY 28，物理相互作用的CaWRKY 40，辣椒免疫力和耐热性的正调节。我们证实了CaWRKY 28-CaWRKY 40相互作用的免疫共沉淀，双分子荧光互补，和微量热泳。我们的研究结果支持了CaWRKY 28是一种核蛋白，在辣椒对病原菌Ralstonia solanacearum感染的反应中起正调控作用的观点。它不是通过直接调节含有免疫相关基因的W盒来发挥其功能，而是通过物理相互作用促进CaWRKY 40结合并激活其免疫相关靶基因，包括CaPR 1，CaNPR 1，CaDEF 1和CaABR 1，但不是其耐热相关靶基因CaHSP 24。所有这些数据表明CaWRKY 28与CaWRKY 40相互作用并增强CaWRKY 40调节针对R的免疫。但不耐热。重要的是，我们发现CaWRKY 28 Cys 249是CaWRKY 28-CaWRKY 40相互作用的关键，CaWRKY 28及其直系同源物可能仅在茄科中共享。这些结果突出了CaWRKY 28如何在WRKY网络的建立过程中与CaWRKY 40相关联，以及CaWRKY 40如何在辣椒对R的响应过程中实现其功能特异性。Solanacearuminfection.版权所有© 2021作者.这是一篇开放获取的文章，在CC BY-NC-ND 4.0国际许可下分发。
WRKY transcription factors have been implicated in plant response to pathogens but how WRKY-mediated networks are organized and operate to produce appropriate transcriptional outputs remains largely unclear. Here, we identify a member of the WRKY family from pepper (Capsicum annuum), CaWRKY28, that physically interacts with CaWRKY40, a positive regulator of pepper immunity and thermotolerance. We confirmed CaWRKY28–CaWRKY40 interaction by coimmunoprecipitation, bimolecular fluorescence complementation, and microscale thermophoresis. Our findings supported the idea that CaWRKY28 is a nuclear protein that acts as positive regulator in pepper responses to infection by the pathogenic bacteriumRalstonia solanacearum. It performs its function not by directly modulating the W-box containing immunity-related genes but by promoting CaWRKY40 via physical interaction to bind and activate its immunity-related target genes, includingCaPR1,CaNPR1,CaDEF1, andCaABR1, but not its thermotolerance-related target gene,CaHSP24. All of these data indicate that CaWRKY28 interacts with and potentiates CaWRKY40 in regulating immunity againstR. solanacearuminfection but not thermotolerance. Importantly, we discovered that CaWRKY28 Cys249, shared by CaWRKY28 and its orthologs probably only in the family Solanaceae, is crucial for the CaWRKY28–CaWRKY40 interaction. These results highlight how CaWRKY28 associates with CaWRKY40 during the establishment of WRKY networks, and how CaWRKY40 achieves its functional specificity during pepper responses toR. solanacearuminfection.Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.