Lead-induced changes of cytoskeletal protein is involved in the pathological basis in mice brain
Lead-induced changes of cytoskeletal protein is involved in the pathological basis in mice brain
复制标题
铅诱导的细胞骨架蛋白变化参与小鼠脑病理基础
DOI：
10.1007/s11356-018-1334-6
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发表时间：
2018-04-01
影响因子：
5.8
通讯作者：
Ning, Hongmei
中科院分区：
文献类型：
--
作者：
Ge, Yaming;Chen, Lingli;Ning, Hongmei
铅中毒是一种地球化学疾病。另一方面，铅具有高度致癌性，对肝脏和肾脏具有毒性。这种元素还可以穿过血脑屏障，降低学习和记忆能力，损害大脑皮层和海马体的结构。为进一步探讨铅的神经毒性机制，采用4周龄昆明种小鼠，研究了不同浓度铅（0、2.4、4.8和9.6 mM）染毒9 d后铅对小鼠神经系统的影响。本研究中，治疗组脑细胞的病理和超微结构变化与线粒体、染色质和细胞核的损伤有关。通过原子吸收光谱法测试了血液中的铅含量，结果显示血液中的铅浓度随着铅剂量的增加而升高。用透射电镜结合能谱分析铅在神经细胞中的分布。结果表明，铅在各染毒组神经细胞核孔、染色质和核膜中均有分布，且铅含量随染毒剂量的增加而增加。最后，通过实时荧光定量PCR和Western blotting检测微管相关蛋白2（MAP2）的mRNA和蛋白表达水平，结果显示MAP2的表达随着铅剂量的增加而降低。这些结果表明，急性铅中毒可引起显着的剂量依赖性毒性作用，对小鼠脑功能，可以有助于更好地了解铅诱导的毒性。
Lead poisoning is a geochemical disease. On the other hand, lead is highly carcinogenic and exhibits liver and kidney toxicity. This element can also cross the blood-brain barrier, reduce learning and memory ability and damage the structure of the cerebral cortex and hippocampus. To further investigate the mechanism of lead neurotoxicity, 4-week-old Kunming mice were used to explore the effects of different concentrations of Pb2+(0, 2.4, 4.8 and 9.6 mM) for 9 days. In this study, pathological and ultrastructural changes in brain cells of the treated group were related to damages to mitochondria, chromatin and the nucleus. Lead content in blood was tested by atomic absorption spectroscopy, which showed high lead concentrations in the blood with increasing doses of lead. Distribution of lead in nerve cells was analysed by transmission electron microscopy with energy dispersive spectroscopy. Data showed the presence of lead in nucleopores, chromatin and nuclear membrane of nerve cells in the treatment groups, whereas lead content increased with increasing doses of lead acetate. Finally, microtubule-associated protein 2 (MAP2) mRNA and protein expression levels were detected by real-time PCR and Western blotting, which showed a reduction in MAP2 expression with increasing lead doses in the mouse brain. These findings suggest that acute lead poisoning can cause significant dose-dependent toxic effects on mouse brain function and can contribute to better understanding of lead-induced toxicity.