Impact of immunization against SpyCEP during invasive disease with two streptococcal species: Streptococcus pyogenes and Streptococcus equi.
Impact of immunization against SpyCEP during invasive disease with two streptococcal species: Streptococcus pyogenes and Streptococcus equi.
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DOI：
10.1016/j.vaccine.2009.06.042
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发表时间：
2009-08-06
期刊：
影响因子：
5.5
通讯作者：
Sriskandan S
中科院分区：
文献类型：
--
作者：
Turner CE;Kurupati P;Wiles S;Edwards RJ;Sriskandan S
目前还没有针对人类病原体化脓性链球菌的许可疫苗。高度保守的IL-8裂解化脓性链球菌细胞膜蛋白SpyCEP在表面表达，是一种潜在的候选疫苗。表达并纯化了重组SpyCEP(CEP)的N-末端部分。抗CEP抗体可中和SpyCEP对IL-8的切割活性。CEP免疫的小鼠减少了局部化脓性链球菌肌肉感染和鼻腔感染的细菌传播。我们还鉴定了马链球菌表达的具有功能的SpyCEP同源蛋白水解酶SeCEP，它能够裂解人和马的IL-8。CEP免疫的小鼠也显示减少了马链球菌肌肉内感染的细菌传播。因此，对SpyCEP的免疫可以提供对其他具有同源酶的链球菌的保护作用。
Currently there is no licensed vaccine against the human pathogen Streptococcus pyogenes. The highly conserved IL-8 cleaving S. pyogenes cell envelope proteinase SpyCEP is surface expressed and is a potential vaccine candidate. A recombinant N-terminal part of SpyCEP (CEP) was expressed and purified. AntiCEP antibodies were found to neutralize the IL-8 cleaving activity of SpyCEP. CEP-immunized mice had reduced bacterial dissemination from focal S. pyogenes intramuscular infection and intranasal infection. We also identified a functional SpyCEP-homolog protease SeCEP, expressed by the equine pathogen Streptococcus equi, which was able to cleave both human and equine IL-8. CEP-immunized mice also demonstrated reduced bacterial dissemination from S. equi intramuscular infection. Therefore immunization against SpyCEP may provide protection against other streptococci species with homologous proteases.
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影响因子：
3.7
作者：
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