Yi-qi-yang-yin-tian-sui-fang enhances cisplatin-induced tumor eradication and inhibits interleukin-7 reduction in non-small cell lung cancer
Yi-qi-yang-yin-tian-sui-fang enhances cisplatin-induced tumor eradication and inhibits interleukin-7 reduction in non-small cell lung cancer
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益气阳阴天随方增强顺铂诱导的非小细胞肺癌肿瘤根除并抑制白细胞介素7减少
DOI：
10.1042/bsr20190052
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发表时间：
2019-06-28
影响因子：
4
通讯作者：
Shi, Lin
中科院分区：
文献类型：
--
作者：
Ke, Bin;Wu, Xinlin;Shi, Lin
中医药在肿瘤治疗的全过程中，被认为有助于提高疗效和减少副作用。中药联合化疗可能通过与白细胞介素-7（IL-7）的协同作用增强机体抗肿瘤免疫应答。本研究以益气养阴天髓方为研究对象，探讨其在非小细胞肺癌（NSCLC）化疗中的作用。用顺铂（DDP）治疗荷瘤小鼠，同时灌胃给予低、中、高剂量的中药（有效含量分别为0.5，2.0和8.0g/只）。结果表明，中药联合用药可进一步提高DDP的疗效，并呈剂量依赖性。Ki-67染色和末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记法（TUNEL）检测肿瘤细胞生长情况。顺铂联合中药治疗能显著降低Ki-67的表达，促进肿瘤细胞凋亡。顺铂可下调IL-7水平，中药可使其恢复。中药还能恢复肿瘤组织中IL-7及其受体IL-7 R的表达。同时观察DDP对骨髓抑制的不良反应。中药能明显抑制DDP诱导的骨髓细胞凋亡，并能显著诱导骨髓IL-7和造血生长因子G-CSF、GM-CSF、SCF、SDF-1的表达。这些数据表明，该中药与DDP联合显示出上级抗肿瘤作用，并通过上调IL-7而减少骨髓抑制。
Traditional Chinese Medicine (TCM) has been recognized to be conducive to enhancing the efficiency and reducing the side effects in the whole course of cancer treatment. The mechanisms of TCM/chemotherapy combination involved with interleukin-7 (IL-7) potentially enhance immune responses against tumor. In the present study, we emphasized on a herbal formulation Yi-qi-yang-yin-tian-sui-fang or TCM for short, and investigated its roles in chemotherapy in non-small cell lung cancer (NSCLC). The mice bared with tumor were treated with cisplatin (DDP) and simultaneously administrated with/without low, medium and high doses of TCMs (effective content: 0.5, 2.0 and 8.0 g/per mice) via oral gavage. The results indicated that combination of TCM further elevated the therapy efficiency of DDP in a dose-dependent manner. The growth of tumor cells was estimated by Ki-67 stain and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) assay. The addition of TCM to the DDP treatment could significantly decrease the expression of Ki-67 and promote the apoptosis of tumor cells. In addition, the serum IL-7 level was down-regulated by DDP but restored by the treatment of TCM. The expression of IL-7 and its receptor IL-7R in tumor tissues was also recovered by TCM. Furthermore, the side effect from bone marrow suppression (myelosuppression) induced by DDP were assessed. TCM could abrogate DDP-induced apoptosis of bone marrow and also remarkably induced the expressions of IL-7 and hematopoietic growth factors including G-CSF, GM-CSF, SCF, and SDF-1 in bone marrow. These data indicated that this TCM combined with DDP showed superior anti-tumor effects with reduced myelosuppression via up-regulating IL-7.