PiggyBac‐like elements in the pink bollworm, Pectinophora gossypiella
PiggyBac‐like elements in the pink bollworm, Pectinophora gossypiella
复制标题
DOI：
10.1111/j.1365-2583.2009.00964.x
复制
发表时间：
2010-04
影响因子：
2.6
通讯作者：
Jianjun Wang;Jianjun Wang;Ernie D. Miller;Gregory S. Simmons;Thomas A. Miller;B. Tabashnik;Yoonseong Park
中科院分区：
文献类型：
--
作者：
Jianjun Wang;Jianjun Wang;Ernie D. Miller;Gregory S. Simmons;Thomas A. Miller;B. Tabashnik;Yoonseong Park
粉红铃虫（Pectinophora gossypiella）（一种重要的鳞翅目棉花害虫）的转基因品系是之前使用来自粉纹夜蛾的piggyBac转座子IFP2产生的。在这里，我们在粉红棉铃虫中鉴定了一种内源性piggyBac样元件（PLE），命名为PgPLE1。假定完整的 PgPLE1 副本 (PgPLE1.1) 呈现出 PLE 的典型特征：末端有 3 个 C/G 残基的反向末端重复序列、反向亚末端重复序列、TTAA 靶位点以及编码转座酶的开放阅读框，与 IFP2 具有 68% 的相似性。 Vectorette PCR 显示全球人群中 PgPLE1 的插入位点存在巨大差异，表明 PgPLE1 的潜在流动性。 PgPLE1 在 Pectinophora endema 的基因组中检测不到，这意味着 PgPLE1 在这两个密切相关的物种分化后最近入侵。
A transgenic line of the pink bollworm, Pectinophora gossypiella, a key lepidopteran cotton pest, was generated previously using the piggyBac transposon IFP2 from Trichoplusia ni. Here we identified an endogenous piggyBac‐like element (PLE), designated as PgPLE1, in the pink bollworm. A putatively intact copy of PgPLE1 (PgPLE1.1) presents the canonical features of PLE: inverted terminal repeats with three C/G residues at the extreme ends, inverted subterminal repeats, TTAA target site and an open reading frame encoding transposase with 68% similarity to IFP2. Vectorette PCR revealed large variation in the insertion sites of PgPLE1 amongst worldwide populations, indicating the potential mobility of PgPLE1. The PgPLE1 was undetectable in the genome of Pectinophora endema, implying the recent invasion of PgPLE1 after the divergence of these two closely related species.