Detection of human papillomavirus DNA in genital warts, cervical dysplasias and neoplasias.
Detection of human papillomavirus DNA in genital warts, cervical dysplasias and neoplasias.
复制标题
检测尖锐湿疣、宫颈发育不良和肿瘤中的人乳头瘤病毒 DNA。
作者：
Y. Tomita;K. Kubota;T. Kasai;S. Sekiya;H. Takamizawa;B. Simizu
采用斑点杂交法和Southern印迹杂交法，在严格的条件下，对日本的尖锐湿疣、鳞状化生、异型增生和宫颈癌等人类生殖器病变组织标本进行了HPV 6、11、16和18相关DNA的筛查。斑点杂交法检测到92.9%(13/14)的尖锐湿疣和6.7%(1/15)的宫颈癌组织中存在HPV6/11相关DNA。宫颈癌组织中HPV16/18相关DNA阳性率为37.7%(5/14)，与浸润性鳞癌和宫颈原位癌的阳性率分别为66.7%(8/12)和50%(9/18)。部分样本DNA进一步用限制性内切酶酶切和Southern印迹分析鉴定，我们证实在一个病变中存在HPV特异性DNA片段，并与HPV6/11和HPV16/18相关的HPV混合感染。
Biopsies from human genital lesions in Japan (108 samples), including condyloma acuminata, squamous metaplasia, dysplasia, and cervical cancer, were screened for the presence of human papillomavirus (HPV) 6-, 11-, 16-, and 18-related DNAs by spot hybridization and Southern blot hybridization under stringent conditions. By spot hybridization, HPV 6/11-related DNA was found in 92.9% (13/14) of condyloma acuminata and in 6.7% (1/15) of cervical cancer biopsies. HPV 16/18-related DNA was found in 37.7% (5/14) of cervical cancer biopsies and was exclusively associated with invasive squamous cell cancer, 66.7% (8/12), and with cervical carcinoma in situ, 50% (9/18). Some sample DNAs were further characterized by restriction enzyme digestion followed by Southern blot analysis, and we confirmed the presence of HPV-specific DNA fragments and mixed infection with both HPV 6/11- and HPV 16/18-related HPVs in one lesion.