Biosynthesis of L-ascorbic acid (vitamin C) by Saccharomyces cerevisiae
Biosynthesis of L-ascorbic acid (vitamin C) by Saccharomyces cerevisiae
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DOI：
10.1016/s0378-1097(00)00155-5
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发表时间：
2000-05-15
影响因子：
2.1
通讯作者：
Viola, R
中科院分区：
文献类型：
--
作者：
Hancock, RD;Galpin, JR;Viola, R
酿酒酵母细胞与D-葡萄糖（D-Glc）、D-半乳糖或D-甘露糖（D-Man）共孵育后，可合成D-赤型抗坏血酸（D-EAA），但不合成L-抗坏血酸（L-AA）。在与D-阿拉伯糖（D-Ara）孵育的细胞中观察到D-EAA蓄积，而在与L-半乳糖（L-Gal）、L-半乳糖酸-1，4-内酯和L-古洛糖酸-1，4-内酯孵育的细胞中观察到L-AA蓄积。当S.将酿酒酵母细胞与D-[U-C-14]Glc、D-[U-C-14]Man或L-[1-C-14]Gal孵育，仅用L-[1-C-14]Gal观察到放射性掺入L-AA。酵母细胞与D-Ara的预孵育基本上减少了L-[1-C-14]Gal掺入L-AA中。我们的研究结果表明，在适当的条件下，酵母细胞可以合成L-AA通过天然用于D-EAA生物合成的途径。(C)2000年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Saccharomyces cerevisiae cells incubated with D-glucose (D-Glc), D-galactose or D-mannose (D-Man) synthesised D-erythroascorbic acid (D-EAA) but not L-ascorbic acid (L-AA). Accumulation of D-EAA was observed in cells incubated with D-arabinose (D-Ara) whilst accumulation of L-AA occurred in cells incubated with L-galactose (L-Gal), L-galaclono-1,4-lactone and L-gulono-1,4-lactone. When S. cerevisiae cells were incubated with D-[U-C-14]Glc, D-[U-C-14]Man or L-[1-C-14]Gal, incorporation of radioactivity into L-AA was observed only with L-[l-C-14]Gal. Pre-incubation of yeast cells with D-Ara substantially reduced the incorporation of L-[l-C-14]Gal into L-AA. Our results indicate that, under appropriate conditions, yeast cells can synthesise L-AA via the pathway naturally used for D-EAA biosynthesis. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.