Comparative transcriptional profiling of Gracilariopsis lemaneiformis in response to salicylic acid- and methyl jasmonate-mediated heat resistance.
Comparative transcriptional profiling of Gracilariopsis lemaneiformis in response to salicylic acid- and methyl jasmonate-mediated heat resistance.
复制标题
龙须菜响应水杨酸和茉莉酸甲酯介导的耐热性的比较转录谱
DOI：
10.1371/journal.pone.0176531
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Sun X
中科院分区：
文献类型：
--
作者：
Wang F;Wang C;Zou T;Xu N;Sun X
由于我国南方沿海夏季气温较高，限制了大型经济植物龙须菜（Gracilariopsis lemaneiformis，红藻门）的栽培。已有研究表明，水杨酸（SA）和茉莉酸甲酯（MJ）两种植物激素可以缓解高温胁迫对银杏的不利影响。柠檬形为了阐明SA和MJ介导的耐热性的分子机制，我们使用RNA测序（RNA-seq）技术对全转录组基因表达谱进行了全面分析。共组装了14，644个unigenes，其中10，501个（71.71%）被注释到参考数据库中。在SA、MJ和SA/MJ处理组中，分别检测到519、830和974个差异表达的单基因。SA富集了与光合作用和糖代谢相关的单基因，而MJ则增加了与糖代谢、蛋白质合成、热激和信号转导相关的单基因。串扰分析显示，216个基因的协同调节，而18个基因的拮抗调节SA和MJ。结果表明，两种植物激素均能通过多种途径缓解热胁迫的不利影响，且以协同作用为主。这些结果将提供新的见解SA和MJ如何调节分子机制，抵消藻类的热应激。
Culturing the economically important macroalga Gracilariopsis lemaneiformis (Rhodophyta) is limited due to the high temperatures in the summertime on the southern Chinese coast. Previous studies have demonstrated that two phytohormones, salicylic acid (SA) and methyl jasmonate (MJ), can alleviate the adverse effects of high-temperature stress on Gp. lemaneiformis. To elucidate the molecular mechanisms underlying SA- and MJ-mediated heat tolerance, we performed comprehensive analyses of transcriptome-wide gene expression profiles using RNA sequencing (RNA-seq) technology. A total of 14,644 unigenes were assembled, and 10,501 unigenes (71.71%) were annotated to the reference databases. In the SA, MJ and SA/MJ treatment groups, 519, 830, and 974 differentially expressed unigenes were detected, respectively. Unigenes related to photosynthesis and glycometabolism were enriched by SA, while unigenes associated with glycometabolism, protein synthesis, heat shock and signal transduction were increased by MJ. A crosstalk analysis revealed that 216 genes were synergistically regulated, while 18 genes were antagonistically regulated by SA and MJ. The results indicated that the two phytohormones could mitigate the adverse effects of heat on multiple pathways, and they predominantly acted synergistically to resist heat stress. These results will provide new insights into how SA and MJ modulate the molecular mechanisms that counteract heat stress in algae.