Integrin beta 3 inhibits hypoxia-induced apoptosis in cardiomyocytes
Integrin beta 3 inhibits hypoxia-induced apoptosis in cardiomyocytes
复制标题
整合素β3抑制缺氧诱导的心肌细胞凋亡
DOI：
10.1093/abbs/gmy056
复制
发表时间：
2018
影响因子：
3.7
通讯作者：
Sun Ting
中科院分区：
文献类型：
--
作者：
Su Yifan;Tian Hua;Wei Lijiang;Fu Guohui;Sun Ting
缺氧诱导的细胞凋亡在心血管疾病中起重要作用。整合素β3是心肌细胞表面主要的整合素异源二聚体受体之一。然而，尽管整合素β3在心血管疾病中发挥重要作用，但其在缺氧反应中的确切作用仍不清楚。因此，本研究旨在研究整合素β3在缺氧诱导的H9C2细胞和原代大鼠心肌细胞凋亡中的作用。MTT法、流式细胞术和TUNEL法结果显示缺氧抑制心肌细胞增殖，诱导心肌细胞凋亡。实时荧光定量PCR和Western blot分析显示，缺氧诱导的心肌细胞中整合素β3和HIF 1 α的表达水平上调。此外，通过siRNA敲低整合素β3表达增加缺氧诱导的心肌细胞凋亡。此外，整合素β3过表达可减弱缺氧诱导的心肌细胞凋亡。急性心肌梗死大鼠心肌组织中整合素β3和HIF 1 α蛋白表达较对照组明显上调。我们的数据表明，整合素β3在缺氧诱导的心肌细胞凋亡中起保护作用。
Hypoxia-induced apoptosis plays an important role in cardiovascular diseases. Integrin β3 is one of the main integrin heterodimer receptors on the surface of cardiac myocytes. However, despite the important role that integrin β3 plays in the cardiovascular disease, its exact role in the hypoxia response remains unclear. Hence, in the present investigation we aimed to study the role of integrin β3 in hypoxia-induced apoptosis in H9C2 cells and primary rat myocardial cells. MTT assay, flow cytometry and TUNEL assay results showed that hypoxia inhibited cardiomyocyte proliferation and induced cardiomyocyte apoptosis. The expression levels of integrin β3 and HIF1α were upregulated in hypoxia-induced cardiomyocytes as revealed by real-time PCR and western blot analysis. Furthermore, knockdown of integrin β3 expression by siRNA increased hypoxia-induced cardiomyocyte apoptosis. In addition, integrin β3 overexpression weakened hypoxia-induced cardiomyocyte apoptosis. The protein expressions of integrin β3 and HIF1α were upregulated in acute myocardial infarction rat cardiac tissues compared with the control rat cardiac tissues. Our data suggest that integrin β3 plays a protective role in cardiomyocytes during hypoxia-induced apoptosis.