The Rice stripe virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana
The Rice stripe virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana
复制标题
水稻条纹病毒pc4在本塞姆氏烟草的运动和叶坏死表达中发挥作用
DOI：
10.1016/j.virol.2012.01.007
复制
发表时间：
2012-04-10
期刊：
影响因子：
3.7
通讯作者：
Li, Weimin
中科院分区：
文献类型：
--
作者：
Zhang, Chao;Pei, Xinwu;Li, Weimin
水稻条纹病毒 (RSV) pc4 已被确定为病毒运动蛋白 (MP)。在这项研究中，pc4 基因被克隆到运动缺陷型烟草花叶病毒 (TMV) 中。由此产生的杂交 TMV-pc4 除了在烟草中进行细胞间传播外，还在烟草中进行系统移动并诱导叶坏死，这表明 RSV MP 的新功能。系统性丙氨酸扫描诱变研究确定了 pc4 的 K-122-D-258 区域与细胞间移动密切相关，并且用丙氨酸替换 KGR(122-124)、D-135、ED170-171、ER201-202、EFE218-220 或 ELD256-258 的突变体不再将细胞移动到细胞。然而，只有一个氨基酸组KGR（122-124）与长距离运动有关。 D-17-K-33 区域被认为是叶坏死反应的关键区域。 DD17-18 或 RK32-33 的诱变大大减轻了坏死。总体数据表明 pc4 在其实验宿主本塞姆氏烟草 RSV 感染过程中发挥多种作用。 (C) 2012 Elsevier Inc. 保留所有权利。
The Rice stripe virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis in Nicotiana benthamiana, indicating novel functions of the RSV MP. A systematic alanine-scanning mutagenesis study established the region K-122-D-258 of the pc4 substantially associated with cell-to-cell movement, and mutants by replacement of KGR(122-124), D-135, ED170-171, ER201-202, EFE218-220 or ELD256-258 with alanine(s) no longer moved cell to cell. However, only one amino acid group KGR(122-124) was linked with long-distance movement. The region D-17-K-33 was recognized as a crucial domain for leaf necrosis response. and mutagenesis of DD17-18 or RK32-33 greatly attenuated necrosis. The overall data suggested manifold roles of the pc4 during the RSV infection in its experimental host N. benthamiana. (C) 2012 Elsevier Inc. All rights reserved.