Isolation and characterization of a wound-inducible ribonuclease from Nicotiana glutinosa leaves.
Isolation and characterization of a wound-inducible ribonuclease from Nicotiana glutinosa leaves.
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从粘性烟草叶中分离和表征伤口诱导核糖核酸酶。
DOI：
10.1271/bbb.62.1144
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Makoto Kimura
中科院分区：
文献类型：
--
作者：
T. Kariu;Kazunari Sano;H. Shimokawa;Riyoko Itoh;Nobuyuki Yamasaki;Makoto Kimura
从烟草（Nicotianaerobiosa）叶片中分离纯化了一种损伤诱导型核糖核酸酶（RNaseNW）。纯化的RNase NW具有约5和7的最佳pH，并且其碱基特异性基于均聚核糖核苷酸的水解的相对速率被建议为鸟嘌呤碱基的偏好。结合蛋白质和cDNA序列分析，推导出RNase NW的完整氨基酸序列。cDNA序列包括具有229个氨基酸的多肽的完整编码序列，包括在N-末端由25个氨基酸组成的推定分泌信号肽。通过蛋白质化学方法鉴定的氨基酸明确地定位在从cDNA序列推导的氨基酸序列内。与其它已知的植物RNase序列比较表明，该RNase NW与N.阿拉塔RNase NE，其在正常条件下存在于花柱和花粉中，并响应于磷酸盐饥饿而在根中被诱导[Dodds等人，厂摩尔生物学，31，227-238（1996）]。RNase NW与其他已知的RNase具有相当大的序列相似性，共有57%至84%的相同残基。以RNase NW cDNA片段为探针的北方印迹分析表明，在未受伤的叶片中未检测到RNase NW转录本，但在受伤后4 h内被诱导，然后在20 h内逐渐减少。
A wound-inducible ribonuclease (RNase NW) was purified from leaves of Nicotiana glutinosa. The purified RNase NW has an optimum pH around 5 and 7, and its base specificity is suggested based on the relative rates of hydrolysis of homopolyribonucleotides to be a preference for guanine base. The complete amino acid sequence of RNase NW was deduced by a combination of protein and cDNA sequencings. The cDNA sequence includes the entire coding sequence for a polypeptide with 229 amino acids including a putative secretion signal peptide at the N-terminus composed of 25 amino acids. The amino acids identified by the protein chemical methods are unambiguously localized within the deduced amino acid sequence from the cDNA sequence. Comparison of the sequence of RNase NW with those of other known plant RNases showed that it was identical except for eight residues to that of N. alata RNase NE, which is present in the style and pollen under normal conditions and is induced in roots in response to phosphate starvation [Dodds et al., Plant. Mol. Biol., 31, 227-238 (1996)]. RNase NW shows considerable sequence similarity to other known RNases, sharing 57% to 84% identical residues. Northern blot analysis using an RNase NW cDNA fragment as a probe showed that the RNase NW transcript was not detected in leaves without wounding, but it was induced within 4 h after wounding and then gradually decreased during 20 h.
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR