SPINDLY is involved in ABA signaling bypassing the PYR/PYLs/RCARs-mediated pathway and partly through functional ABAR
SPINDLY is involved in ABA signaling bypassing the PYR/PYLs/RCARs-mediated pathway and partly through functional ABAR
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DOI：
10.1016/j.envexpbot.2018.04.004
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发表时间：
2018-07-01
影响因子：
5.7
通讯作者：
Fan, Liu-Min
中科院分区：
文献类型：
--
作者：
Liang, Lin;Yang, Jing;Fan, Liu-Min
SPINDLY（SPY）是一种O-连接的[3-N-乙酰葡糖胺转移酶和O-岩藻糖基转移酶，参与多种信号传导途径。我们报道了SPY参与阿坝信号转导，spy突变体在种子萌发和幼苗早期发育中表现出对阿坝不敏感的表型。SPY与ABA介导的种子萌发和早期幼苗发育的关键转录因子ABI 5、ABI 3和ABI 4之间存在复杂的关系。这种关系依赖于发育阶段。在种子萌发和子叶变绿方面，四倍体突变体雌spy-3、雄pyl 1 - 1、pyl 4 -1、p在RNA-seq分析中，与Col-0相比，spy-3在阿坝下萌发种子的总体转录水平上调。spy-3中ABAR的转录水平上调，而另外两种阿坝受体ABAR和GTG 1/GTG 2的转录水平基本保持不变。遗传分析表明，spy-3对阿坝的不敏感性受到ABAR突变体cch的阻碍，表现为种子萌发和子叶变绿，反之亦然。遗传分析还表明，SPY通过ABAR调控叶绿体功能和叶片发育。总之，我们得出结论，SPY参与阿坝信号转导绕过PYR介导的途径，但可能发挥作用的阿坝信号转导部分通过ABAR介导的途径。
SPINDLY (SPY) is a putative O-linked [3-N-acetyl glucosamine transferase and an O-fucosyltransferase, involved in various signaling pathways. We report here that SPY is involved in ABA signaling, spy mutants have insensitive phenotypes in response to ABA in seed germination and early seedling development. SPY has a complex relationship with ABI5, ABI3 and ABI4, which are key transcription factors in ABA-mediated seed germination and early seedling development. This relationship is dependent of developmental stage. Quadruple mutants female spy-3 male pyl1-1 pyl4-1 pyr1-1 and female pyl1-1pyl4-1 pyr1-1 male spy-3 display enhanced insensitivity to ABA compared with either pyl1-1/pyl4-1/pyr1-1 or spy-3 in terms of seed germination and cotyledon greening. In RNA-seq assay, the general transcription level of germinating seeds of spy-3 under ABA is up-regulated compared with that of Col-0. The transcription level of ABAR , another ABA receptor spanning the chloroplast envelope, is up-regulated in spy-3 whereas another two types of putative ABA receptors ABAR and GTG1/GTG2 remain largely unaltered, As uncovered by genetic analyses, insensitivity to ABA of spy-3 is hindered by cch, a mutant of ABAR, in terms of seed germination and cotyledon greening, and vice versa. Genetic analyses also reveal that SPY regulates chloroplast function and leaf development via ABAR. Altogether, we conclude that SPY is involved in ABA signaling bypassing PYR-mediated pathway but likely playing a role in ABA signaling partly via the ABAR-mediated pathway.