Localized cerebral blood flow response at submillimeter columnar resolution
Localized cerebral blood flow response at submillimeter columnar resolution
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DOI：
10.1073/pnas.191101098
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发表时间：
2001-09-11
影响因子：
11.1
通讯作者：
Kim, SG
中科院分区：
文献类型：
--
作者：
Duong, TQ;Kim, DS;Kim, SG
功能磁共振成像(FMRI)已被广泛应用于脑功能成像。然而，在亚毫米分辨率下，活动部位周围的fMRI血流动力学反应的范围仍然知之甚少，也存在争议。使用基于血流灌注的功能磁共振成像，我们评估了猫视觉皮质在定向刺激后的血流动力学反应。使用脑血流fMRI测量获得的激活图主要没有大的引流静脉污染，并且在柱状分辨率下可重现。刺激特异性脑血流反应在空间上定位于单独的皮质柱，并解析柱状布局。定向柱状结构的周期间距估计为1.1+/-0.2 mm(n=14个定向，5只动物)，与以前的发现一致。在无差分减影的单刺激条件下，半峰全宽时的脑血流反应估计为470微米。这些结果表明，如果可以最大限度地减少或消除大血管的贡献，基于血流动力学的功能磁共振确实可以用于标测单个功能柱。
Functional magnetic resonance imaging (fMRI) has been widely used for imaging brain functions. However, the extent of the fMRI hemodynamic response around the active sites, at submillimeter resolution, remains poorly understood and controversial. With the use of perfusion-based fMRI, we evaluated the hemodynamic response in the cat visual cortex after orientation-specific stimuli. Activation maps obtained by using cerebral blood flow fMRI measurements were predominantly devoid of large draining vein contamination and reproducible at columnar resolution. Stimulus-specific cerebral blood flow responses were spatially localized to individual cortical columns, and columnar layouts were resolved. The periodic spacing of orientation columnar structures was estimated to be 1.1 +/- 0.2 mm (n = 14 orientations, five animals), consistent with previous findings. The estimated cerebral blood flow response at full width at half-maximum was 470 mum under single-stimulus conditions without differential subtraction. These results suggest that hemodynamic-based fMRI can indeed be used to map individual functional columns if large-vessel contributions can be minimized or eliminated.