A SPECTRAL CHARACTERIZATION OF THE LIGHT-HARVESTING PIGMENT-PROTEIN COMPLEXES FROM RHODOPSEUDOMONAS-ACIDOPHILA
A SPECTRAL CHARACTERIZATION OF THE LIGHT-HARVESTING PIGMENT-PROTEIN COMPLEXES FROM RHODOPSEUDOMONAS-ACIDOPHILA
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DOI：
10.1016/0005-2728(86)90208-2
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发表时间：
1986-03-12
期刊：
影响因子：
--
通讯作者：
HIPKINS, MF
中科院分区：
文献类型：
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作者：
ANGERHOFER, A;COGDELL, RJ;HIPKINS, MF
从两株野生型嗜酸红假单胞菌制备了4种不同的天线配合物，并记录了它们在280 ~ 4 K温度范围内的吸收光谱、荧光发射光谱和激发光谱。在每种情况下，随着温度的降低，荧光产率增加。测定了在每个复合物内从类胡萝卜素到细菌叶绿素a的单线态-单线态能量转移的效率。该过程的效率与温度无关。有趣的是，根据复合物的类型，类胡萝卜素到细菌叶绿素a能量转移的相对效率显著不同。有人认为，这种差异可能在一定程度上解释了为什么Rps。当嗜酸菌在不同的光强下生长时，它会改变细胞中存在的可变天线成分的类型。
Four different antenna-complexes were prepared from two wild-type strains of Rhodopseudomonas acidophila and their absorption, fluorescence emission and excitation spectra were recorded as a function of temperature in the range from 280 K to 4 K. In each case as the temperature was lowered the fluorescence yield increased. The efficiency of singlet-singlet energy transfer from the carotenoids to the bacteriochlorophyll a within each complex was determined. The efficiency of this process was shown to be temperature independent. Interestingly the relative efficiency of the carotenoid-to-bacteriochlorophyll a energy transfer varied markedly, depending upon the type of complex. It is suggested that this variation may, in part, explain why Rps. acidophila alters the type of the variable antenna component present in its cells when it is grown at different light intensities.