MACROPHAGE-MEDIATED MYELIN-RELATED MITOGENIC FACTOR FOR CULTURED SCHWANN-CELLS
MACROPHAGE-MEDIATED MYELIN-RELATED MITOGENIC FACTOR FOR CULTURED SCHWANN-CELLS
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DOI：
10.1073/pnas.85.5.1701
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发表时间：
1988-03-01
影响因子：
11.1
通讯作者：
DEVRIES, GH
中科院分区：
文献类型：
--
作者：
BAICHWAL, RR;BIGBEE, JW;DEVRIES, GH
来自已吞噬髓磷脂膜部分的培养的腹膜巨噬细胞的条件培养基对于培养的许旺细胞是促有丝分裂的。促有丝分裂上清液的产生是时间和剂量依赖性的，在将培养的巨噬细胞与富含髓鞘的级分（200 μ g蛋白质/ml）孵育48小时后，来自上清液的最大许旺细胞增殖应答。反应是特定的髓鞘膜：从巨噬细胞与轴膜，肝微粒体，聚苯乙烯珠，或脂多糖孵育的上清液不促有丝分裂。髓鞘膜的溶酶体加工对于促有丝分裂因子的产生是必要的，促有丝分裂因子被证明是热不稳定的和胰蛋白酶敏感的。没有种属特异性，因为从大鼠、牛和人的中枢和外周神经系统中分离的髓鞘膜在诱导有丝分裂上清液中具有同等效力。然而，来自中枢神经系统髓鞘膜的上清液比从相同物种的外周神经系统组分获得的上清液的促有丝分裂性高两到三倍。以前的观察，髓磷脂是有丝分裂的培养许旺细胞可能，部分涉及的中间处理髓磷脂的巨噬细胞中存在的许旺细胞培养物。这些结果表明，巨噬细胞在华勒变性过程中雪旺细胞增殖中起着至关重要的作用。
Conditioned medium from cultured peritoneal macrophages that have phagocytosed a myelin membrane fraction is mitogenic for cultured Schwann cells. Production of the mitogenic supernatant was time- and dose-dependent with a maximal Schwann cell-proliferative response from supernatants after 48-hr incubation of cultured macrophages with myelin-enriched fraction (200 .mu.g of protein per ml). The response was specific for myelin membrane: supernatants derived from macrophages incubated with axolemma, liver microsomes, polystyrene beads, or lipopolysaccharide were not mitogenic. Lysosomal processing of the myelin membrane was necessary for the production of the mitogenic factor, which was shown to be heat labile and trypsin sensitive. There was no species specificity because myelin membranes isolated from the central and peripheral nervous systems of rat, bovine, and human were equally potent in eliciting mitogenic supernatant. However, supernatants derived from central nervous system myelin membranes were two to three times more mitogenic than those obtained from peripheral nervous system fractions of the same species. Previous observations that myelin is mitogenic for cultured Schwann cells may, in part, involve the intermediate processing of myelin by macrophages that are present in Schwann cell cultures. These results suggest that macrophages play a crucial role in Schwann cell proliferation during Wallerian degeneration.