Nanoscopic Cellular Imaging: Confinement Broadens Understanding.
Nanoscopic Cellular Imaging: Confinement Broadens Understanding.
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DOI：
10.1021/acsnano.6b02863
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发表时间：
2016-09
期刊：
影响因子：
17.1
通讯作者：
Stephen A. Lee;A. Ponjavic;C. Siv;Steven F. Lee;J. Biteen
中科院分区：
文献类型：
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作者：
Stephen A. Lee;A. Ponjavic;C. Siv;Steven F. Lee;J. Biteen
近年来，单分子荧光成像一直在调和光学显微镜和亚细胞生物物理学之间的根本不匹配。然而，在活细胞中的纳米级成像的下一步只能通过光学激发限制几何形状来访问。在这里，我们回顾了三种限制方法，可以使纳米级成像在活细胞：激发限制激光照明与光束整形;物理限制细菌细胞中的微米级几何形状;和纳米光子学的纳米级限制。
In recent years, single-molecule fluorescence imaging has been reconciling a fundamental mismatch between optical microscopy and subcellular biophysics. However, the next step in nanoscale imaging in living cells can be accessed only by optical excitation confinement geometries. Here, we review three methods of confinement that can enable nanoscale imaging in living cells: excitation confinement by laser illumination with beam shaping; physical confinement by micron-scale geometries in bacterial cells; and nanoscale confinement by nanophotonics.