Identification of two functional forms of immunoglobulin G3-binding protein expressed by group A streptococci.
Identification of two functional forms of immunoglobulin G3-binding protein expressed by group A streptococci.
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A 组链球菌表达的免疫球蛋白 G3 结合蛋白的两种功能形式的鉴定。
DOI：
10.1128/iai.62.5.2104-2107.1994
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发表时间：
1994
影响因子：
3.1
通讯作者：
Boyle,MD
中科院分区：
文献类型：
--
作者：
Pack,TD;Otten,RA;Raeder,RH;Boyle,MD
A组链球菌免疫球蛋白G （IgG）结合蛋白与不同的人igg3 -骨髓瘤蛋白的反应性分析为这些分子的至少两种功能形式提供了证据。分离具有代表性的igg3结合分子，进行生物素化处理，并用作竞争结合试验的示踪剂。交叉抑制研究表明存在两种不同的igg3结合活性模式。链球菌蛋白G可以抑制一种形式的蛋白与igg3 -骨髓瘤蛋白的结合，而第二种形式的结合则不受抑制。这些研究进一步强调了A组链球菌表达的免疫球蛋白结合蛋白的异质性程度。
Analysis of group A streptococcal immunoglobulin G (IgG)-binding protein reactivity with different human IgG3-myeloma proteins provided evidence for at least two functional forms of these molecules. Representative IgG3-binding molecules were isolated, biotinylated, and used as tracers in competitive binding assays. Cross-inhibition studies demonstrated the existence of two distinct patterns of IgG3-binding activity. Proteins of one form could be inhibited from binding to an IgG3-myeloma protein by streptococcal protein G while binding of the second form was not inhibited. These studies further underscore the extent of heterogeneity among immunoglobulin-binding proteins expressed by group A streptococci.