Efflux pump lde is associated with fluoroquinolone resistance in Listeria monocytogenes
Efflux pump lde is associated with fluoroquinolone resistance in Listeria monocytogenes
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DOI：
10.1128/aac.47.2.704-708.2003
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发表时间：
2003-02-01
影响因子：
4.9
通讯作者：
Courvalin, P
中科院分区：
文献类型：
--
作者：
Godreuil, S;Galimand, M;Courvalin, P
在488株李斯特菌的耐药性筛选中，发现5株单核细胞增生李斯特菌（CLIP 21369、CLIP 73298、CLIP 74811、CLIP 75679和CLIP 79372）对氟喹诺酮类药物耐药。从法国的人类淋病病例中分离的单核细胞增多症菌株。在利血平存在下环丙沙星MIC降低四倍或更多的基础上，氟喹诺酮类耐药归因于药物的主动外排。lde基因（李斯特菌药物外排，以前的lmo2741）编码一个12跨膜节段推定的外排泵，属于二级转运蛋白的主要促进者超家族，与肺炎链球菌的PmrA具有44%的同一性。CLIP 21369中lde基因的插入失活表明，相应的蛋白质负责氟喹诺酮类药物耐药性，并参与对溴化乙锭和吖啶橙子等染料的敏感性水平。
Five Listeria monocytogenes isolates (CLIP 21369, CLIP 73298, CLIP 74811, CLIP 75679, and CLIP 79372) were found to be resistant to fluoroquinolones during the screening for antibiotic resistance of 488 L. monocytogenes isolates from human cases of listeriosis in France. On the basis of a fourfold or greater decrease in the ciprofloxacin MIC in the presence of reserpine, fluoroquinolone resistance was attributed to active efflux of the drugs. The lde gene (Listeria drug efflux; formerly lmo2741) encodes a 12-transmembrane-segment putative efflux pump belonging to the major facilitator superfamily of secondary transporters that displayed 44% identity with PmrA from Streptococcus pneumoniae. Insertional inactivation of the lde gene in CLIP 21369 indicated that the corresponding protein was responsible for fluoroquinolone resistance and was involved in the level of susceptibility to dyes such as ethidium bromide and acridine orange.