Concise Review: Mesenchymal Stem Cells for Functional Cartilage Tissue Engineering: Taking Cues from Chondrocyte-Based Constructs.
Concise Review: Mesenchymal Stem Cells for Functional Cartilage Tissue Engineering: Taking Cues from Chondrocyte-Based Constructs.
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DOI：
10.1002/sctm.16-0271
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发表时间：
2017-04
影响因子：
6
通讯作者：
Hung CT
中科院分区：
文献类型：
--
作者：
Tan AR;Hung CT
骨关节炎是关节疾病中最常见的形式，困扰着9%的30岁以上的美国人口，每年在医疗保健和社会经济成本方面花费近1000亿美元。其特征在于关节疼痛和功能障碍，尽管病理生理学仍然在很大程度上未知。由于其无血管性质和有限的细胞结构，关节软骨在损伤后表现出较差的内在愈合反应。因此，重要的研究工作旨在生产工程软骨作为关节软骨修复的基于细胞的方法。然而，膝关节在机械上要求很高，并且在受伤期间，也是苛刻的炎症介质的环境。无情的机械化学环境需要能够承受这种负担的组织替代品。间充质干细胞（MSC）用于软骨组织工程已经成为一种有前途的细胞来源，因为它们易于分离，在培养中容易扩增的能力，以及经历谱系特异性分化成软骨细胞的能力。然而，迄今为止，很少有利用MSC的研究成功地概括了天然软骨的结构和功能特性，暴露了在体外培养期间和体内植入后将干细胞均匀分化成所需细胞命运并保持表型的困难过程。为了解决这些缺点，在这里，我们对使用基于软骨细胞的软骨组织工程的成熟技术调节干细胞行为、组织发育和功能进行了简要回顾。干细胞转化医学2017;6：1295-1303
Osteoarthritis, the most prevalent form of joint disease, afflicts 9% of the U.S. population over the age of 30 and costs the economy nearly $100 billion annually in healthcare and socioeconomic costs. It is characterized by joint pain and dysfunction, though the pathophysiology remains largely unknown. Due to its avascular nature and limited cellularity, articular cartilage exhibits a poor intrinsic healing response following injury. As such, significant research efforts are aimed at producing engineered cartilage as a cell‐based approach for articular cartilage repair. However, the knee joint is mechanically demanding, and during injury, also a milieu of harsh inflammatory agents. The unforgiving mechano‐chemical environment requires tissue replacements that are capable of bearing such burdens. The use of mesenchymal stem cells (MSCs) for cartilage tissue engineering has emerged as a promising cell source due to their ease of isolation, capacity to readily expand in culture, and ability to undergo lineage‐specific differentiation into chondrocytes. However, to date, very few studies utilizing MSCs have successfully recapitulated the structural and functional properties of native cartilage, exposing the difficult process of uniformly differentiating stem cells into desired cell fates and maintaining the phenotype during in vitro culture and after in vivo implantation. To address these shortcomings, here, we present a concise review on modulating stem cell behavior, tissue development and function using well‐developed techniques from chondrocyte‐based cartilage tissue engineering. Stem Cells Translational Medicine 2017;6:1295–1303