Identification of metabolites of trenbolone acetate in androgenic runoff from a beef feedlot.
Identification of metabolites of trenbolone acetate in androgenic runoff from a beef feedlot.
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从牛肉饲养场中鉴定乙酸三酚乙酸酯的代谢产物。
DOI：
10.1289/ehp.8055
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发表时间：
2006-04
影响因子：
10.4
通讯作者：
Ankley, Gerald T
中科院分区：
文献类型：
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作者：
Durhan, Elizabeth J;Lambright, Christy S;Makynen, Elizabeth A;Lazorchak, James;Hartig, Phillip C;Wilson, Vickie S;Gray, L Earl;Ankley, Gerald T
关于动物饲养业排放物中的生物活性物质的潜在生态影响，人们知之甚少。醋酸群勃龙是一种合成的合成代谢类固醇，在美国被广泛用于促进肉牛的生长。醋酸群勃龙的代谢产物包括立体异构体17α-和17β-群勃龙，这两种物质在动物粪便中稳定，在鱼类和哺乳动物中是相对有效的雄激素。本研究的目的是评价肉牛饲养场排放物和排放物上游和下游河水中17α-和17β-群勃龙的发生率。在这方面的努力，我们测量了在体外雄激素活性的放电使用CV-1细胞，已被瞬时共转染与人类雄激素受体和报告基因的结构。在2002年和2003年期间，在9个不同的场合收集了样本。排放的全水样品在CV-1细胞中引起显著的雄激素反应，并含有可检测浓度的17α-和17β-群勃龙。需要进一步的工作，以确定在何种程度上合成雄激素，如群勃龙有助于雄激素活性的饲养场放电。
Little is known concerning the potential ecological effects of hormonally active substances associated with discharges from animal feeding operations. Trenbolone acetate is a synthetic anabolic steroid that is widely used in the United States to promote growth of beef cattle. Metabolites of trenbolone acetate include the stereoisomers 17α- and 17β-trenbolone, both of which are stable in animal wastes and are relatively potent androgens in fish and mammals. Our purpose in this study was to evaluate the occurrence of 17α- and 17β-trenbolone in a beef cattle feedlot discharge and in river water upstream and downstream from the discharge. In conjunction with that effort, we measured in vitro androgenic activity of the discharge using CV-1 cells that had been transiently cotransfected with human androgen receptor and reporter gene constructs. Samples were collected on nine different occasions during 2002 and 2003. Whole-water samples from the discharge caused a significant androgenic response in the CV-1 cells and contained detectable concentrations of 17α- and 17β-trenbolone. Further work is needed to ascertain the degree to which synthetic androgens such as trenbolone contribute to androgenic activity of feedlot discharges.