Membrane heterogeneity in murine stem cells
Membrane heterogeneity in murine stem cells
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小鼠干细胞的膜异质性
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
A. Norman
中科院分区：
文献类型：
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作者：
C. P. Myers;D. Kerk;A. Norman
细胞膜在造血分化和调控中的作用日益明显。某些荧光分子与膜组分在分子水平上相互作用。有机染料花菁540(MCN)就是在分子水平上研究造血干细胞膜的工具。本文介绍了MCN与慢性淋巴细胞白血病(CLL)和造血细胞膜的生物相互作用。作者的工作表明，MNC染色揭示了伴随细胞转化和分化的膜差异。MNC荧光的变化表明，造血细胞的膜特性是异质性的，而对MNC光失活的敏感性表明，膜特性可能随着多能和定向粒细胞前体细胞之间的分化状态而改变。
The cell membrane's role in hemopoietic differentiation and regulation is increasingly evident. Certain fluorescent molecules interact with membrane components at the molecular level. The organic dye merocyanine 540(MCN) is such a tool for studying hemopoietic stem cell membranes at the molecular level. This paper present work involving biologic interactions of MCN with CLL (chronic lymphocytic leukemia) and hemopoietic cell membranes. The authors work indicates that MNC staining reveals membrane differences that accompany cellular transformation and differentiation. Changes in MNC fluorescene indicate that hemopoietic cells are heterogeneous with respect to membrane properties, and sensitivity to MNC photoinactivation reveals that membrane properties may change as a function of differentiative state between pluripotent and committed granulocytic precursor cells.