Synthetic evolving systems that implement a user-specified genetic code of arbitrary design.
Synthetic evolving systems that implement a user-specified genetic code of arbitrary design.
复制标题
实现用户指定的任意设计的遗传密码的合成进化系统。
DOI：
10.1016/j.chembiol.2012.08.017
复制
发表时间：
2012
影响因子：
--
通讯作者：
Joyce,GeraldF
中科院分区：
文献类型：
--
作者：
Sczepanski,JonathanT;Joyce,GeraldF
基于交叉复制RNA酶的合成遗传系统提供了一种评估替代遗传密码的方法，这些遗传密码将遗传信息与相应的分子功能联系起来。编码组合化学的一个特殊的实现被用来构建复杂的人口的交叉复制的RNA酶根据用户指定的代码，涉及基因型和表型的分子的基础上。复制酶经历了自我维持的达尔文进化，导致了最有利变体的出现。这些酶既包括维持整个种群的高活性酶，也包括作为活性分子寄生虫存活的低活性酶。这种进化结果是遗传密码的信息容量和保真度的结果，这表明应该如何调整这些参数以实现针对特定应用的代码。
A synthetic genetic system, based on cross-replicating RNA enzymes, provides a means to evaluate alternative genetic codes that relate heritable information to corresponding molecular function. A special implementation of encoded combinatorial chemistry was used to construct complex populations of cross-replicating RNA enzymes in accordance with a user-specified code that relates genotype and phenotype on a molecule-by-molecule basis. The replicating enzymes were made to undergo self-sustained Darwinian evolution, resulting in the emergence of the most advantageous variants. These included both highly active enzymes that sustained the population as a whole and poorly active enzymes that survived as parasites of the active molecules. This evolutionary outcome was a consequence of the information capacity and fidelity of the genetic code, suggesting how these parameters should be adjusted to implement codes tailored to particular applications.