IDENTIFICATION OF A LIGAND FOR THE C-KIT PROTOONCOGENE
IDENTIFICATION OF A LIGAND FOR THE C-KIT PROTOONCOGENE
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DOI：
10.1016/0092-8674(90)90297-r
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发表时间：
1990-10-05
期刊：
影响因子：
64.5
通讯作者：
LYMAN, SD
中科院分区：
文献类型：
--
作者：
WILLIAMS, DE;EISENMAN, J;LYMAN, SD
我们报道了一种新的肥大细胞生长因子，称为MGF，从小鼠基质细胞系的上清液的纯化和n端氨基酸序列。在[3H]胸苷掺入试验中，筛选了一组白细胞介素3依赖性细胞系对部分纯化的MGF的反应性；MGF对这些细胞的增殖刺激与c-kit原癌基因mRNA的表达相关。通过将125i标记的MGF交联到表达c-kit的细胞上，随后用c-kit的c端特异性抗血清免疫沉淀复合物，MGF被证明是c-kit的配体。这证实了MGF是c-kit蛋白的配体。
We report the purification and N-terminal amino acid sequence of a novel mast cell growth factor, termed MGF, from the supernatants of a murine stromal cell line. A panel of interleukin 3-dependent cell lines were screened for responsiveness to partially purified MGF in [3H]thymidine incorporation assays; proliferative stimulation of these cells in response to MGF correlated with expression of mRNA for the c-kit proto-oncogene. MGF was shown to be a ligand for c-kit by cross-linking 125I-labeled MGF to c-kit-expressing cells with subsequent immunoprecipitation of the complex with antiserum specific for the C-terminus of c-kit. This establishes MGF as a ligand for the c-kit protein.