Expanded bed adsorption/desorption of proteins with Streamline Direct CST I adsorbent
Expanded bed adsorption/desorption of proteins with Streamline Direct CST I adsorbent
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DOI：
10.1002/bit.20952
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发表时间：
2006-08
影响因子：
3.8
通讯作者：
Ping Li;G. Xiu;Vera G. Mata;Carlos A. Grande;A. Rodrigues
中科院分区：
文献类型：
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作者：
Ping Li;G. Xiu;Vera G. Mata;Carlos A. Grande;A. Rodrigues
Streamline Direct CST I是一种新型的具有多模式功能基团的离子交换剂，专为膨胀床吸附（EBA）工艺而设计，可直接从高离子强度原料中捕获具有高结合能力的蛋白质。在本研究中，在填充有Streamline Direct CST I的膨胀床中进行了双组分蛋白质（BSA和肌红蛋白）竞争吸附和解吸的实验研究。基于Streamline Direct CST I上单组分和双组分牛血清白蛋白（BSA）/肌红蛋白吸附等温线的测量，选择了整个EBA过程的结合和洗脱条件;然后进行了较长时间尺度的前沿分析和固定床柱位移实验（XK 16/20色谱柱），以评价Streamline Direct CST I上双组分蛋白质（BSA和肌红蛋白）的竞争性吸附和置换。最后，在填充有300 mL Streamline Direct CST I的Streamline 50色谱柱中讨论了通过填充有Streamline Direct CST I的膨胀床捕获BSA和肌红蛋白的可行性。© 2006 Wiley Periodicals，Inc.
Streamline Direct CST I is a new type of ion exchanger with multi‐modal functional groups, specially designed for an expanded bed adsorption (EBA) process, which can capture directly the proteins from the high ionic strength feedstocks with a high binding capacity. In this study, an experimental study is carried out for two‐component proteins (BSA and myoglobin) competitive adsorption and desorption in an expanded bed packed with Streamline Direct CST I. Based on the measurements of the single‐ and two‐component bovine serum albumin (BSA)/myoglobin adsorption isotherm on Streamline Direct CST I, the binding and elution conditions for the whole EBA process are selected; and then frontal analysis for a longer timescale and column displacement experiments in a fixed bed (XK16/20 column) are carried out to evaluate the two‐component proteins (BSA and myoglobin) competitive adsorption and displacement on Streamline Direct CST I. Finally, the feasibility of capturing both BSA and myoglobin by an expanded bed packed with Streamline Direct CST I is addressed in a Streamline 50 column packed with 300 mL Streamline Direct CST I. © 2006 Wiley Periodicals, Inc.