The crayfish Procambarus clarkii CRY shows daily and circadian variation
The crayfish Procambarus clarkii CRY shows daily and circadian variation
复制标题
DOI：
10.1242/jeb.00900
复制
发表时间：
2004-04-01
影响因子：
2.8
通讯作者：
Hernández-Herrera, G
中科院分区：
文献类型：
--
作者：
Fanjul-Moles, ML;Escamilla-Chimal, EG;Hernández-Herrera, G
小龙虾的昼夜节律是由蓝光通过假定的视网膜外光感受器引起的。我们调查的存在和日常变化的CRY，一种蛋白质光敏蓝光光谱和无处不在的动物和植物，在推定的心脏起搏器的克氏原螯虾，即眼柄和大脑，在不同的时间的24小时光：暗周期。使用不同的实验光协议，并通过定性/定量免疫荧光解剖和生化方法，我们确定CRY免疫反应性细胞位于延髓-半椭圆体复合体（MT-HB）和前缘的中前脑（PR）。免疫反应随时间变化，两种神经结构呈半镜像。生化分析的结果与这些变化相匹配。蛋白质印迹法显示脑CRY丰度具有统计学显著的昼夜节律，但眼柄中没有昼夜CRY丰度振荡。这些免疫细胞化学和生物化学结果将特定的感光分子与昼夜节律联系起来。我们认为，CRY可能与生物钟的光感受性和昼夜节律的产生有关。
The circadian rhythms of crayfish are entrained by blue light, through putative extra retinal photoreceptors. We investigated the presence and daily variation of CRY, a protein photosensitive to blue light spectra and ubiquitous in animals and plants, in the putative pacemakers of Procambarus clarkii, namely the eyestalk and brain, at different times of the 24 h light:dark cycles. Using different experimental light protocols and by means of qualitative/quantitative immunofluorescence anatomical and biochemical methods, we identified CRY inummoreactivity in cells located in the medullaterminalis-hemiellipsoidal complex (MT-HB) and the anterior margin of the median protocerebrum (PR). The immunoreaction varied with the time of day and the two neural structures showed a semi-mirror image. The results of the biochemical analysis matched these variations. Western blotting demonstrated statistically significant circadian rhythms in brain CRY abundance, but no daily circadian CRY abundance oscillations in the eyestalk. These immunocytochemical and biochemical results link a specific photoreceptor molecule to circadian rhythmicity. We propose that CRY may be linked to the photoreception of the clock and to the generation of circadian rhythmicity.